Skip to content
2000
Volume 13, Issue 1
  • ISSN: 2211-5366
  • E-ISSN: 2211-5374

Abstract

Background: Alteration in the expression and activity of drug-metabolizing enzymes (DMEs) can alter the pharmacokinetics and hence the response of the drug. Some chemicals found in herbs and fruits affect the expression of DMEs. is commonly used in Middle Eastern Arabic countries. There is no report regarding the influence of on the hepatic expression of DMEs. Aims: The current investigation aimed to investigate the effect of consumption on the mRNA expression of major hepatic drug-metabolizing cytochrome (cyp) P450 genes in mice. Methods: The chemical composition of the ethanoic extract was analyzed using liquid chromatography/ mass spectrometry. Then, 21 BALB/c mice were used for the in vivo experiment. The mice were divided into three groups, each consisting of seven mice. The first group (low-dose group) was treated with 41.6 mg/kg of extract and the second group was administered the high-dose (125 mg/kg) of the extract for one month. The mice in the third “control” group administrated the vehicle 20% polyethylene glycol 200. Then, the expression of cyp3a11, cyp2c29, cyp2d9, and cyp1a1 was analyzed using the real-time polymerase chain reaction. The relative liver weights of the mice and the hepatic pathohistological alterations were assessed. Results: The ethanolic extract of contained 27 phytochemical compounds. The most abundant compounds were linolenic acid, myristic acid, and p-cymene. It was found that the low dose of extract upregulated significantly (P < 0.05) the expression of cyp3a11 by more than ten folds in the liver of treated mice. Furthermore, the histological analysis showed that low- and high-dose administration of the C. incana did not cause pathological alterations. Conclusion: It can be concluded from these findings that consumption of low doses of Calamintha incana upregulated the mRNA expression of mouse cyp3a11 without causing histopathological alterations in the livers. Further studies are needed to determine the influence of on the pharmacokinetics and response of drugs metabolized by cyp3a11.

Loading

Article metrics loading...

/content/journals/mirna/10.2174/0122115366268781231205103752
2024-03-01
2025-08-16
Loading full text...

Full text loading...

/content/journals/mirna/10.2174/0122115366268781231205103752
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test