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image of Evaluation of Anti-epileptic Activity of Cyanthillium cinereum (L.) H. Rob. Leaves in the Experimental Pentylenetetrazole-induced Epileptic Model

Abstract

Background

Epilepsy is a common and frequently devastating disorder affecting millions of people. According to a recent survey, 1-2% of the Indian population suffers from major mental disorders and 5% suffers from minor mental disorders. Epilepsy is among those mental disorders that affect 30 million people worldwide. Currently, the treatment of epilepsy involves agents which modulate sodium-ion channels, enhance GABAergic transmission, and agents with multiple modes of action. Various classes of synthetic drugs are used to treat epilepsy, but these drugs are often challenged due to their unwanted side effects. Medicinal plants have been a part of human society which combating diseases from the dawn of civilization. The plant (L.) H. Rob. is mainly found in the Himalayas from Kashmir to Nepal at an altitude of 8000 m. Decoction of this plant is traditionally used as an anti-cancer, anti-malarial, anti-epileptic, and in neurosis and skin diseases.

Objectives

The present study investigated the anti-epileptic activity of leaves against pentylenetetrazole (PTZ)-induced epileptic model in mice.

Methods

Plant extracts were prepared using solvents in increasing polarity viz., petroleum ether, chloroform, ethanol, and water, using a Soxhlet apparatus. The bio-active extract was characterized using FTIR and GC techniques. antioxidants like GSH and SOD level, oxidative stress markers- MDA and hemoglobin and platelet count were also estimated in the animal brain.

Results

Amongst all extracts tested, only ethanol extract of significantly (p<0.05) inhibited generalized tonic-clonic seizures in PTZ-induced epilepsy in mice in a dose (100 or 200 mg/kg., p.o.) dependent manner. The dose of 200 mg/kg of extract exhibited the most significant effect. It is also found that treatment with ethanol extract on PTZ-induced epilepsy in mice significantly (p<0.05) reduces the duration of convulsion and delays the onset of clonic convulsion.

Conclusion

The present findings suggest that the high amounts of phenols and flavonoids in the ethanol extract could be responsible for the anti-epileptic effect. Moreover, the ethanol extract also restored GSH, SOD and hemoglobin and platelet level and decreased oxidative marker- MDA content in the mice brain.

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2025-05-27
2025-09-27

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References

  1. Falco-Walter J. Epilepsy—Definition, classification, pathophysiology, and epidemiology. Semin. Neurol. 2020 40 6 617 623 10.1055/s‑0040‑1718719 33155183
    [Google Scholar]
  2. O’Donohoe T.J. Choudhury A. Callander E. Global macroeconomic burden of epilepsy and the role for neurosurgery: A modelling study based upon the 2016 global burden of disease data. Eur. J. Neurol. 2020 27 2 360 368 10.1111/ene.14085 31549472
    [Google Scholar]
  3. Surguchov A. Surgucheva I. Sharma M. Sharma R. Singh V. Pore-forming proteins as mediators of novel epigenetic mechanism of epilepsy. Front. Neurol. 2017 8 3 3 10.3389/fneur.2017.00003 28149289
    [Google Scholar]
  4. Lasoń W. Chlebicka M. Rejdak K. Research advances in basic mechanisms of seizures and antiepileptic drug action. Pharmacol. Rep. 2013 65 4 787 801 10.1016/S1734‑1140(13)71060‑0 24145073
    [Google Scholar]
  5. Wolf P. History of epilepsy: Nosological concepts and classification. Epileptic Disord. 2014 16 3 261 269 10.1684/epd.2014.0676 25256654
    [Google Scholar]
  6. Gilani A.H. Aziz N. Khan M.A. Shaheen F. Jabeen Q. Siddiqui B.S. Herzig J.W. Ethnopharmacological evaluation of the anticonvulsant, sedative and antispasmodic activities of Lavandula stoechas L. J. Ethnopharmacol. 2000 71 1-2 161 167 10.1016/S0378‑8741(99)00198‑1 10904159
    [Google Scholar]
  7. Dogra N.K. Kumar S. A review on ethno-medicinal uses and pharmacology of Vernonia cinerea Less. Nat. Prod. Res. 2015 29 12 1102 1117 10.1080/14786419.2014.981814 25426778
    [Google Scholar]
  8. Chen X. Zhan Z.J. Yue J.M. Sesquiterpenoids from Vernonia cinerea. Nat. Prod. Res. 2006 20 1 31 35 10.1080/14786410500078276 16286305
    [Google Scholar]
  9. Bagheri S.M. Sahebkar A. Gohari A.R. Saeidnia S. Malmir M. Iranshahi M. Evaluation of cytotoxicity and anticonvulsant activity of some Iranian medicinal Ferula species. Pharm. Biol. 2010 48 3 242 246 10.3109/13880200903081796 20645807
    [Google Scholar]
  10. Farnsworth N.R. Biological and phytochemical screening of plants. J. Pharm. Sci. 1966 55 3 225 276 10.1002/jps.2600550302 5335471
    [Google Scholar]
  11. Mendhekar S.Y. Balsaraf C.D. Bangar M.S. Jadhav S.L. Gaikwad D.D. Pharmacognostic, phytochemical, physicochemical and TLC profile study Mace (Aril) of Myristica malabarica Lamk. (Myristicaceae). Journal of Phytopharmacology 2017 6 6 329 334 10.31254/phyto.2017.6604
    [Google Scholar]
  12. Shah S.R. Ukaegbu C.I. Hamid H.A. Alara O.R. Evaluation of antioxidant and antibacterial activities of the stems of Flammulina velutipes and Hypsizygus tessellatus (white and brown var.) extracted with different solvents. J. Food Meas. Charact. 2018 12 3 1947 1961 10.1007/s11694‑018‑9810‑8
    [Google Scholar]
  13. C R. As V. K N. A review: Cyanthillium cinereum (L) H. Rob. Int. J. Res. Rev. 2021 8 9 99 101 10.52403/ijrr.20210914
    [Google Scholar]
  14. Brunetti D. Torsvik J. Dallabona C. Teixeira P. Sztromwasser P. Fernandez-Vizarra E. Cerutti R. Reyes A. Preziuso C. D’Amati G. Baruffini E. Goffrini P. Viscomi C. Ferrero I. Boman H. Telstad W. Johansson S. Glaser E. Knappskog P.M. Zeviani M. Bindoff L.A. Defective PITRM 1 mitochondrial peptidase is associated with Aβ amyloidotic neurodegeneration. EMBO Mol. Med. 2016 8 3 176 190 10.15252/emmm.201505894 26697887
    [Google Scholar]
  15. Tzoulis C. Bindoff L.A. Acute mitochondrial encephalopathy reflects neuronal energy failure irrespective of which genome the genetic defect affects. Brain 2012 135 12 3627 3634 10.1093/brain/aws223 23065482
    [Google Scholar]
  16. Kaneko K. Itoh K. Berliner L.J. Miyasaka K. Fujii H. Consequences of nitric oxide generation in epileptic‐seizure rodent models as studied by in vivo EPR. Magn. Reson. Med. 2002 48 6 1051 1056 10.1002/mrm.10297 12465116
    [Google Scholar]
  17. Gutteridge J.M.C. Rowley D.A. Halliwell B. Superoxide-dependent formation of hydroxyl radicals and lipid peroxidation in the presence of iron salts. Detection of ‘catalytic’ iron and anti-oxidant activity in extracellular fluids. Biochem. J. 1982 206 3 605 609 10.1042/bj2060605 6293469
    [Google Scholar]
  18. Kakkar P. Das B. Viswanathan P.N. A modified spectrophotometric assay of superoxide dismutase. Indian J. Biochem. Biophys. 1984 21 2 130 132 6490072
    [Google Scholar]
/content/journals/cnsamc/10.2174/0118715249352799250512015642
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Evaluation of Anti-epileptic Activity of Cyanthillium cinereum (L.) H. Rob. Leaves in the Experimental Pentylenetetrazole-induced Epileptic Model
Bentham Science Publishers ; https://doi.org/10.2174/0118715249352799250512015642
/content/journals/cnsamc/10.2174/0118715249352799250512015642
/content/journals/cnsamc/10.2174/0118715249352799250512015642
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  • Article Type:     Research Article
Keywords: phenolic and flavonoid compounds ; antiepileptic ; Cyanthillium cinereum ; antioxidant

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