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2000
Volume 25, Issue 12
  • ISSN: 1566-5240
  • E-ISSN: 1875-5666

Abstract

Background

This study focuses on exploring the impact of Astragaloside IV [AS-IV] on osteogenic differentiation.

Methods

Osteogenic differentiation was induced in rat osteoblasts, following which treatment with AS-IV at varied doses was performed. Using Alizarin red staining and alkaline phosphatase (ALP) detection assay, the osteogenic differentiation of the cells was investigated. The expressions of osteogenic differentiation-related genes were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway-associated protein expressions were examined using Western blot. After osteoblasts were transfected with protein tyrosine phosphatase non-receptor type 2 (PTPN2) overexpression plasmid, the impact of PTPN2 on osteoblasts treated with AS-IV was examined.

Results

AS-IV treatment enhanced osteogenic differentiation and up-regulated the expression of osteogenic differentiation-related genes, as well as the levels of p-PI3K/PI3K and p-AKT/AKT, while reducing phosphatase and tensin homolog (PTEN) protein production in osteoblasts. Overexpression of PTEN inhibited osteogenic differentiation, and PTPN2 overexpression counteracted the effects of AS-IV on osteogenic differentiation.

Conclusion

AS-IV contributing to osteogenic differentiation may be related to the PTPN2-mediated PTEN/PI3K/Akt pathway.

© 2025 Bentham Science Publishers
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2025-02-12
2025-12-16

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Mechanism of Astragaloside IV in Promoting Osteogenic Differentiation
Current Molecular Medicine 25, 1507 (2025); https://doi.org/10.2174/0115665240333454241203050356
/content/journals/cmm/10.2174/0115665240333454241203050356
/content/journals/cmm/10.2174/0115665240333454241203050356
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  • Article Type:     Research Article
Keyword(s): astragaloside IV; Osteogenic differentiation; protein tyrosine phosphatase non-receptor type 2; PTEN; PTEN/PI3K/Akt signaling pathway; qRT-PCR

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