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2000
Volume 22, Issue 5
  • ISSN: 1570-1638
  • E-ISSN: 1875-6220

Abstract

Objective/Introduction

This study investigated the antimicrobial properties of the thanatin peptide against oral bacteria associated with dental caries and endodontic failures. Additionally, the cytotoxic effects of this peptide on human gingival fibroblast cells (HGFCs) were assessed.

Methods and Materials

The antimicrobial property of thanatin was tested on , , , and , using the microbroth dilution method. The 0.2% Chlorhexidine mouthwash was used as the control group. Additionally, the cytotoxicity was measured using the MTT assay. The results were presented descriptively and analyzed one-way ANOVA and Tukey's HSD tests.

Results and Discussion

Thanatin demonstrated the strongest bacteriostatic effect (MIC) against , measuring 4.68 μg/ml, which is approximately double that of and , with concentrations of 9.37 and 8.75 μg/ml, respectively. The highest bactericidal activity (MBC) of thanatin was noted in and at 9.37 μg/ml. The antibacterial effects of thanatin against evaluated bacteria were several times lower than those of Chlorhexidine. The cytotoxicity assessment indicated that over 70% and 60% of the HGFCs remained viable after 24 and 48 hours, respectively.

Conclusion

Although thanatin exhibited significantly higher biocompatibility, its antimicrobial effectiveness against the tested oral bacteria was inferior to that of 0.2% Chlorhexidine.

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2025-02-24
2025-09-25

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Evaluation of Cytotoxicity and Antimicrobial Activity of Thanatin Recombinant Peptide against Some Oral Bacteria: A Novel Approach against Bacterial Pathogens in Dentistry
Current Drug Discovery Technologies 22, e15701638332473 (2025); https://doi.org/10.2174/0115701638332473250213064453
/content/journals/cddt/10.2174/0115701638332473250213064453
/content/journals/cddt/10.2174/0115701638332473250213064453
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  • Article Type:     Research Article
Keyword(s): Antimicrobial peptide; chlorhexidine; Enterococcus faecalis; Streptococcus mutans; Streptococcus oralis; Streptococcus salivarius; thanatin

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