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2000
Volume 25, Issue 2
  • ISSN: 1871-5249
  • E-ISSN: 1875-6166

Abstract

Background

Audiogenic Epilepsy (AEs) is a subtype of epileptic seizure that is generally caused by high-intensity sounds. A large number of traditional medicines has been explored in this lieu where our study chased L. (Rubiaceae), an herbal plant which is commonly known as Lady's Bedstraw, that contains a highly rich chemical composition including flavonoids (Hispidulin, Quercetin, and Kaempferol), and phenolic acids (chlorogenic acid, caftaric acid, and gallic acid). is well known for its antioxidant, neuroprotective, and anti-inflammatory properties. Recently, the unique role of Adhesion G Protein-Coupled Receptor V1 (ADGRV1) protein in the progression of audiogenic epilepsy has been explored.

Aims and Objectives

This study aimed to examine the potent phytoconstituents of the hydroalcoholic extract of (HEGV) using analytical techniques. Additionally, our study sought to evaluate the antioxidant, neuroprotective, anti-inflammatory properties, and antiepileptic potency of HEGV by targeting ADGRV1 and analyses using SH-SY5Y cells.

Methods

HPLC and LC-MS techniques were employed to identify the flavonoids, iridoids, and phenolic acid derivatives present in HEGV. DPPH (2,2-diphenyl-1-picrylhydrazyl), nitric oxide (NO), and hydroxyl (OH) radical scavenging assays were performed to confirm the antioxidant potential of the extract. Additionally, molecular docking and molecular dynamic studies were performed using AutoDock Vina software to analyze the possible interactions between crucial phytoconstituents of HEGV and ADGRV1, followed by cell line analysis. In the analysis, antioxidant, neuroprotective, and anti-inflammatory properties were assessed cell viability assay, IL, GABA, and glutamate estimation.

Results

LC-MS and HPLC analyses revealed high concentrations of hispidulin, a major flavonoid found in HEGV. HEGV exhibited moderate-to-high free radical-scavenging activities comparable to those of ascorbic acid. Docking analysis demonstrated that hispidulin has a stronger binding affinity with ADGRV1 (Vina score = -8.6 kcal/mol) than other compounds. Furthermore, cell line analysis revealed that the MSG exacerbates the neurodegeneration and neuroinflammation, whereas, HEGV and Hispidulin both possess neuroprotective, antioxidant, and antiepileptic activities.

Conclusion

HEGV and Hispidulin proved to be promising candidates for treating audiogenic epilepsy by modulating ADGRV1.

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Investigating the Therapeutic Property of Galium verum L. (GV) for MSG induced Audiogenic Epilepsy (AEs) and Neuroprotection through In-silico and In-vitro Analysis
Central Nervous System Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry - Central Nervous System Agents) 25, 181 (2025); https://doi.org/10.2174/0118715249330123240822063420
/content/journals/cnsamc/10.2174/0118715249330123240822063420
/content/journals/cnsamc/10.2174/0118715249330123240822063420
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  • Article Type:     Research Article
Keyword(s): ADGRV1; Audiogenic epilepsy (AEs); Galium verum; hispidulin; in silico analysis; in-vitro analysis; neurodegeneration

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