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2000
Volume 1, Issue 1
  • ISSN: 2210-299X
  • E-ISSN: 2210-3007
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Abstract

Background

Glucose regulation and energy homeostasis mitigate energy crises milieu in reperfusion injury. We investigated Banaba for its outcomes on cerebral ischemia reperfusion (IR) injury using artery occlusion in rats. The pleiotropic activity of Banaba on various debilitating mechanisms inducing reperfusion injury was evaluated.

Aim

This study aimed to evaluate the pharmacological activity of Banaba () extract on reperfusion injury and investigate the effect of Banaba on vascular permeability, oxidative stress and cellular damage in ischemia reperfusion injury in rats.

Methods

Transient ischemia and reperfusion through occlusion of the middle cerebral artery (MCAO) lead to Cerebral IR injury in Wistar rats; it was treated with oral administration of Banaba extract (100mg/kg and 200mg/kg). The injury outcomes were evaluated after 22 hours of reperfusion by determining cellular injury, its impact on musculoskeletal coordination, multiple free radical scavenging measures (SOD, GSH, LPO) and vascular permeability of the blood-brain barrier.

Results

Banaba treatment led to a marked improvement in neurological outcomes by enhanced coordination and reduced cerebral infarct in comparison to vehicle control ischemic group. Free radical scavenging activity (SOD and GSH) was significantly better, and lipid peroxidation was reduced by Banaba treatment; it also reduced the vascular permeability of the blood-brain barrier. We observed that a lower dose of Banaba (100 mg/kg) was more effective than the higher (200 mg/kg) in ischemic rats. The anti-inflammatory and anti-oxidant activity could drive the neuroprotective outcomes of Banaba in cerebral IR injury. The critical factor of the beneficial effect of Banaba in cerebral injury is the optimization of dose in this experimental setup of reperfusion injury using rats.

Conclusion

The recovery of injury could be attributed to Banaba’s multi-factorial effect targeting free radicals, inflammation, and necrosis during ischemia-reperfusion injury.

© 2024 The Author(s). Published by Bentham Science Publisher.
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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2023-11-07
2025-09-16

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References

  1. ThiyagarajanM. KaulC.L. SharmaS.S. Neuroprotective efficacy and therapeutic time window of peroxynitrite decomposition catalysts in focal cerebral ischemia in rats #.Br. J. Pharmacol.2004142589991110.1038/sj.bjp.070581115197101
     [Google Scholar]
  2. PhamM. HelluyX. KleinschnitzC. KraftP. BartschA.J. JakobP. NieswandtB. BendszusM. StollG. Sustained reperfusion after blockade of glycoprotein-receptor-Ib in focal cerebral ischemia: An MRI study at 17.6 Tesla.PLoS One201164e1838610.1371/journal.pone.001838621483769
     [Google Scholar]
  3. YangC. WangH. ZhangS. ChengY. SunJ. Neuroprotective effects of NKN on focal cerebral ischemia in rats.Turk Neurosurg.20122211622274963
     [Google Scholar]
  4. JonnalaR.R. BuccafuscoJ.J. Inhibition of nerve growth factor signaling by peroxynitrite.J. Neurosci. Res.2001631273410.1002/1097‑4547(20010101)63:1<27::AID‑JNR4>3.0.CO;2‑#11169611
     [Google Scholar]
  5. DhoteV. BalaramanR. Anti-oxidant activity mediated neuroprotective potential of trimetazidine on focal cerebral ischaemia-reperfusion injury in rats.Clin. Exp. Pharmacol. Physiol.2008355-663063710.1111/j.1440‑1681.2008.04845.x18318745
     [Google Scholar]
  6. ChenS.D. YangD.I. LinT.K. ShawF.Z. LiouC.W. ChuangY.C. Roles of oxidative stress, apoptosis, PGC-1α and mitochondrial biogenesis in cerebral ischemia.Int. J. Mol. Sci.201112107199721510.3390/ijms1210719922072942
     [Google Scholar]
  7. KleinG. KimJ. HimmeldirkK. CaoY. ChenX. Antidiabetes and anti-obesity activity of Lagerstroemia speciosa.Evid. Based Complement. Alternat. Med.20074440140710.1093/ecam/nem01318227906
     [Google Scholar]
  8. Rohit SinghT. EzhilarasanD. Lagerstroemia speciosa (L.) Pers., ethanolic extract attenuates simultaneously administered isoniazid‐ and dapsone‐induced hepatotoxicity in rats.J. Food Biochem.2021458e1383010.1111/jfbc.1383034155655
     [Google Scholar]
  9. YamadaK. HosokawaM. YamadaC. WatanabeR. FujimotoS. FujiwaraH. KunitomoM. MiuraT. KanekoT. TsudaK. SeinoY. InagakiN. Dietary corosolic acid ameliorates obesity and hepatic steatosis in KK-Ay mice.Biol. Pharm. Bull.200831465165510.1248/bpb.31.65118379057
     [Google Scholar]
  10. ParkC. LeeJ.S. Banaba: The natural remedy as antidiabetic drug.Biomed. Res.2011222125129
     [Google Scholar]
  11. DhoteV. BalaramanR. RajaM.K. Cardioprotective effect of banaba on myocardial ischemia/reperfusion injury in rats.J. Nat. Rem.202014014810.18311/jnr/2020/25156
     [Google Scholar]
  12. ChomovaM. ZitnanovaI. Look into brain energy crisis and membrane pathophysiology in ischemia and reperfusion.Stress201619434134810.1080/10253890.2016.117484827095435
     [Google Scholar]
  13. ReichmannH. MalteseW.A. DeVivoD.C. Enzymes of fatty acid β-oxidation in developing brain.J. Neurochem.198851233934410.1111/j.1471‑4159.1988.tb01044.x2899130
     [Google Scholar]
  14. LongaEZ WeinsteinPR CarlsonS CumminsR Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke19892018491
     [Google Scholar]
  15. GhoneimA. Abdel-NaimA.B. KhalifaA. El-DensharyE.S. Protective effects of curcumin against ischaemia/reperfusion insult in rat forebrain.Pharmacol. Res.200246327327910.1016/S1043‑6618(02)00123‑812220971
     [Google Scholar]
  16. BedersonJB PittsLH TsujiM NishimuraMC DavisRL BartkowskiH Rat middle cerebral artery occlusion: Evaluation of the model and development of a neurologic examination.Stroke1986173472476
     [Google Scholar]
  17. MisraH.P. FridovichI. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase.J. Biol. Chem.1972247103170317510.1016/S0021‑9258(19)45228‑94623845
     [Google Scholar]
  18. MoronMS DepierreJW MannervikB Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver.Biochim. Biophys. Acta.197958216778
     [Google Scholar]
  19. SlaterT.F. SawyerB.C. The stimulatory effect of carbon tetrachloride and other halogenoalkanes or peroxidative reactions in rat liver fractions in vitro.Biochem. J.1971123580581410.1042/bj12308054399399
     [Google Scholar]
  20. Gürsoy-ÖzdemirY. BolayH. SaribaşO. DalkaraT. Role of endothelial nitric oxide generation and peroxynitrite formation in reperfusion injury after focal cerebral ischemia.Stroke20003181974198110.1161/01.STR.31.8.197410926966
     [Google Scholar]
  21. LowryO. RosebroughN. FarrA.L. RandallR. Protein measurement with the Folin phenol reagent.J. Biol. Chem.1951193126527510.1016/S0021‑9258(19)52451‑614907713
     [Google Scholar]
  22. BaiN. HeK. RollerM. ZhengB. ChenX. ShaoZ. PengT. ZhengQ. Active compounds from Lagerstroemia speciosa, insulin-like glucose uptake-stimulatory/inhibitory and adipocyte differentiation-inhibitory activities in 3T3-L1 cells.J. Agric. Food Chem.20085624116681167410.1021/jf802152z19053366
     [Google Scholar]
  23. LiuX. KimJ. LiY. LiJ. LiuF. ChenX. Tannic acid stimulates glucose transport and inhibits adipocyte differentiation in 3T3-L1 cells.J. Nutr.2005135216517110.1093/jn/135.2.16515671208
     [Google Scholar]
  24. VijaykumarK. MurthyP.B. KannababuS. SyamasundarB. SubbarajuG.V. Quantitative determination of corosolic acid in Lagerstroemia speciosa leaves, extracts and dosage forms.Int. J. Appl. Sci. Eng.200642103114
     [Google Scholar]
  25. LiJ. QiY. LiuH. CuiY. ZhangL. GongH. LiY. LiL. ZhangY. Acute high-altitude hypoxic brain injury: Identification of ten differential proteins.Neural Regen. Res.20138312932294125206614
     [Google Scholar]
  26. ZhouX. LiC. XuW. ChenJ. Trimetazidine protects against smoking-induced left ventricular remodeling via attenuating oxidative stress, apoptosis, and inflammation.PLoS One201277e4042410.1371/journal.pone.004042422792312
     [Google Scholar]
  27. AlmeidaA. AlmeidaJ. BolañosJ.P. MoncadaS. Different responses of astrocytes and neurons to nitric oxide: The role of glycolytically generated ATP in astrocyte protection.Proc. Natl. Acad. Sci.20019826152941529910.1073/pnas.26156099811742096
     [Google Scholar]
  28. NowakP. ZagziłT. KoneckiJ. SzczerbakG. SzkilnikR. NiwińskiJ. GorzałekJ. KostrzewaR.M. BrusR. Trimetazidine increases [3H]glucose uptake in rat brain.Pharmacol. Rep.200658455956116963803
     [Google Scholar]
  29. YuL. YangB. WangJ. ZhaoL. LuoW. JiangQ. YangJ. Time course change of COX2-PGI2/TXA2 following global cerebral ischemia reperfusion injury in rat hippocampus.Behav. Brain Funct.2014101425210.1186/1744‑9081‑10‑4225388440
     [Google Scholar]
  30. FangJ. HolmgrenA. Inhibition of thioredoxin and thioredoxin reductase by 4-hydroxy-2-nonenal in vitro and in vivo.J. Am. Chem. Soc.200612861879188510.1021/ja057358l16464088
     [Google Scholar]
  31. KawaboriM. YenariM. Inflammatory responses in brain ischemia.Curr. Med. Chem.201522101258127710.2174/092986732266615020915403625666795
     [Google Scholar]
  32. RinkC. KhannaS. Significance of brain tissue oxygenation and the arachidonic acid cascade in stroke.Antioxid. Redox Signal.201114101889190310.1089/ars.2010.347420673202
     [Google Scholar]
  33. HinsonH.E. RowellS. SchreiberM. Clinical evidence of inflammation driving secondary brain injury.J. Trauma Acute. Care Surg.201578118419110.1097/TA.000000000000046825539220
     [Google Scholar]
  34. MousaA.M. El-SammadN.M. Abdel-HalimA.H. AnwarN. KhalilW.K.B. NawwarM. HashimA.N. ElsayedE.A. HassanS.K. Lagerstroemia speciosa (L.) Pers leaf extract attenuates lung tumorigenesis via alleviating oxidative stress, inflammation and apoptosis.Biomolecules201991287189210.3390/biom912087131842482
     [Google Scholar]
  35. PriyaT.T. SabuM.C. JollyC.I. Free radical scavenging and anti-inflammatory properties of Lagerstroemia speciosa (L).Inflammopharmacology200816418218710.1007/s10787‑008‑7002‑618759076
     [Google Scholar]
  36. Sai SaraswathiV. KamarudheenN. BhaskaraRaoK.V. SanthakumarK. Phytoremediation of dyes using Lagerstroemia speciosa mediated silver nanoparticles and its biofilm activity against clinical strains Pseudomonas aeruginosa.J. Photochem. Photobiol. B201716810711610.1016/j.jphotobiol.2017.02.00428212517
     [Google Scholar]
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Banaba Restricts Brain Damage: Neuroprotective Role in Cerebral Ischemia-reperfusion Injury
Curr. Indian Sci. 1, E2210299X261536 (2023); https://doi.org/10.2174/012210299X261536231025093606
/content/journals/cis/10.2174/012210299X261536231025093606
/content/journals/cis/10.2174/012210299X261536231025093606
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  • Article Type:     Research Article
Keyword(s): Banaba; Cerebral ischemia; Free radicals; Inflammation; Middle cerebral artery occlusion; Reperfusion injury

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