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2000
Volume 16, Issue 2
  • ISSN: 2772-574X
  • E-ISSN: 2772-5758

Abstract

Aim

Quantification using an HPLC-FLD based detector and Molecular identification of Ochratoxin-A producing Species isolated from stored grain samples.

Background

Fungi are cosmopolitan in origin and are known to grow in any suitable substratum. In the present investigation, species isolated from stored grain samples were analyzed for ochratoxin-A production.

Objective

The objective of this study is the quantification of Ochratoxin and identification of ochratoxigenic fungi.

Methods

A total of n=34 black and n=1 Ochre were isolated from grain samples of Bihar, India and it was tested for OTA production. The limit of detection (LOD) is found to be 0.33 µg/kg and the limit of quantification (LOQ) is found to be 1 µg/kg for OTA in HPLC-FLD.

Results

In the present study, out of all the fungal isolates, only TiB fungal isolate was able to produce the ochratoxin-A above the level of LOQ. The positive isolate TiB obtained from stored sesame seed samples was able to produce 25.54 µg/kg of OTA. ITS sequence analysis of TiB isolate was able to match 100% with and .

Conclusion

This is the initial report of ochratoxigenic fungal isolate isolated from stored sesame seed samples of flood-prone areas of Bihar, India.

© 2025 Bentham Science Publishers
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Quantification using a HPLC-FLD-based Detector and Molecular Identification of Ochratoxin-A Producing Aspergillus Species Isolated from Stored Grain Samples
Recent Pat Food Nutr Agric 16, 223 (2025); https://doi.org/10.2174/012772574X302254240610094258
/content/journals/rafna/10.2174/012772574X302254240610094258
/content/journals/rafna/10.2174/012772574X302254240610094258
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  • Article Type:     Research Article
Keyword(s): Aspergillus foetidus; Aspergillus welwitschiae; fungi; HPLC-FLD; ITS sequencing; Ochratoxin-A

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