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2000
Volume 15, Issue 6
  • ISSN: 2210-3155
  • E-ISSN: 2210-3163

Abstract

Background

There is a research gap about applying titania–graphene oxide (GO-TiO) nanocomposite and coronatine as elicitors in the cell suspension culture of .

Objective

The effects of two elicitors of GO-TiO (30 µg/L) and coronatine (10 µM) were examined to improve the production of five different taxanes in the cell suspension culture of .

Methods

The suspension culture was prepared from the callus of stems explant. Next, both elicitors of coronatine (10 µM) and GO-TiO (30 µg/L) were applied, sampling occurred 2, 4, 16 days after elicitation, and all five taxanes were quantified using HPLC.

Results

The amounts of 10-DAB III ranged from 0.84 mg/L (for control) to 9.17 mg/L (day 16 after elicitation with GO-TiO). The production of BAC III ranged from zero (for control) to 7.69 mg/L (day 4 with GO-TiO). The maximum production of 10-deacetyltaxol was observed in control on day 16 with the amount of 1.85 mg/L, followed by a lower level of 0.825 mg/L on day 2 with coronatine treatment. Considering cephalomannine, only elicitation of GO-TiO over all three different sampling times (., days 2, 4, and 16) resulted in sensible quantities. The maximum magnitudes of taxol were acquired when GO-TiO was applied on day 4 and day 16 (1.345 mg/L and 0.965 mg/L, respectively).

Conclusion

The results indicated the potential positive effects of both elicitors, particularly GO-TiO nanocomposite, to improve taxanes production.

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Improvement of Taxane Biosynthesis in Cell Suspension Culture of Taxus baccata after Elicitation with Titania-graphene Oxide Nanocomposite and Coronatine
Natural Products Journal, The 15, E030724231509 (2025); https://doi.org/10.2174/0122103155282541240624050103
/content/journals/npj/10.2174/0122103155282541240624050103
/content/journals/npj/10.2174/0122103155282541240624050103
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  • Article Type:     Research Article
Keyword(s): chemotherapy; coronatine; taxanes; taxol; Taxus baccata; TiO2/graphene nanocomposite

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