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2000
Volume 20, Issue 3
  • ISSN: 1574-8928
  • E-ISSN: 2212-3970

Abstract

Background

Oxidative stress refers to non-homeostatic elevation within intracellular reactive oxygen species (ROS) levels and is associated with several neuro-related pathological conditions. Diclofenac is a commonly prescribed non-steroidal anti-inflammatory drug (NSAID) for treating aches and pain by reducing inflammation. Diclofenac is also associated with the induction of apoptotic cell death by altering the homeostatic balance within mitochondria. In the present report, the neuroprotective effects of BNC formulation constituted by leaves, and rhizome seeds were investigated.

Methods

The synthesized formulation was characterized using FT-IR and LC-MS along with organoleptic evaluation. Thereafter neuroprotective efficacy of BNC formulation was subsequently investigated against Diclofenac-induced oxidative stress in SH-SY5Y cells. The cells were pretreated with synthesized formulation and subsequently evaluated for amelioration in Diclofenac-induced cytotoxicity, and ROS augmentation. The neuroprotective effect of synthesized formulation was further explored by evaluating the changes in nuclear morphology, and apoptosis alleviation with concomitant regulatory effects on caspase-3 and -9 activation.

Results

Diclofenac was found to be considerably cytotoxic against human neuroblastoma SH-SY5Y cells. Intriguingly, Diclofenac-mediated toxicity was reduced significantly in SH-SY5Y cells pretreated with BNC formulation. Augmented ROS levels within Diclofenac-treated SH-SY5Y cells were also reduced in the BNC formulation pretreated SH-SY5Y cells. Furthermore, BNC formulation pretreated SH-SY5Y cells also exhibited reduced dissipation of mitochondrial membrane potential, caspase-3 and -9, along with apoptosis after Diclofenac treatment.

Conclusion

These findings indicated that, indeed, Diclofenac induces considerable ROS-mediated apoptosis in SH-SY5Y cells, which further intriguingly ameliorated Diclofenac-mediated cytotoxic effects on SH-SY5Y cells. This manuscript further collected information about available National and International patents published or granted in preparation of and thereof applications against motor and non-motor brain dysfunctions.

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2025-09-21

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Protective Assessment of Novel (BNC Formulation) against Brain Tumor
Recent Patents on Anti-Cancer Drug Discovery 20, 460 (2025); https://doi.org/10.2174/0115748928272753231212043701
/content/journals/pra/10.2174/0115748928272753231212043701
/content/journals/pra/10.2174/0115748928272753231212043701
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  • Article Type:     Research Article
Keyword(s): Bacopa monneiri; Curcuma longa; neuroblastoma; neuroprotective; Nigella sativa; SH-SY5Y

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