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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Hydroxyapatite Elution Behavior of Human Nucleotide Excision Repair Protein XPA and Fragments of XPA
- Source: Protein and Peptide Letters, Volume 8, Issue 5, Oct 2001, p. 357 - 365
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- 01 Oct 2001
Abstract
The hydroxyapatite elution behavior of 3 fragments of human XPA (M98-F219 = XPA-MBD M59-F219 = XPA-EM M59-M273 = XPA-deltaN58) and full-length human XPA containing a 6-residue, N-terminal histidine tag (h6-XPA) are described. With increasing concentration of potassium phosphate the proteins elute in the order: XPA-EM, XPA-MBD, XPA-deltaN58, and h6-XPA. If hydroxyapatite affinity is related to DNA-binding affinity, then h6-XPA and XPA-deltaN58 may bind DNA more tightly than XPA-MBD and XPA-EM.
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