Isolation and Identification of Two Membrane Proteins Binding to Transfer RNA

Quan-Sheng Liu; Jian-Hua Liu; De-Sheng Jiang; You-Xin Jin; De-Bao Wang

doi:10.2174/0929866013409265

ISSN: 0929-8665
E-ISSN: 1875-5305

Isolation and Identification of Two Membrane Proteins Binding to Transfer RNA
Authors: Quan-Sheng Liu¹, Jian-Hua Liu², De-Sheng Jiang³, You-Xin Jin⁴ and De-Bao Wang⁵
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¹ State Key Laboratory of Molecular Biology, the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China ² State Key Laboratory of Molecular Biology, the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China ³ State Key Laboratory of Molecular Biology, the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China ⁴ State Key Laboratory of Molecular Biology, the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China ⁵ State Key Laboratory of Molecular Biology, the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Source: Protein and Peptide Letters, Volume 8, Issue 5, Oct 2001, p. 367 - 374
DOI: https://doi.org/10.2174/0929866013409265
- Available online: 01 Oct 2001

Abstract

Two major tRNA binding proteins were obtained by biochemical method from yeast crude extracts of total membrane proteins. They were further confirmed to be tRNA specific-binding proteins by gel mobility shift assay. N-terminal sequence assays showed that they were glyceraldehyde-3-phosphate dehydranase (GAPDH) and 3-phosphate glycerate kinase (PGK1), respectively.

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2001-10-01

2025-09-01

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Article Type: Review Article

Keyword(s): glyceraldehyde-3-phosphate dehydranase (GAPDH); RNA polymerase III

Isolation and Identification of Two Membrane Proteins Binding to Transfer RNA

Abstract

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