Natural Products Journal, The - Online First

The complex etiology of Gouty Arthritis (GA) makes it a multidimensional illness for which there are now no effective therapeutic therapies that can stop its development entirely. This review examines how effectively different plants and their constituents reduce blood uric acid levels and lessen the predisposition to gout. Research has looked at the possible roles of oxidative stress markers, inflammatory pathways, important transport and signaling proteins, and the health benefits of different plants and their components in the development of GA. Plant remedies have been used for a long time to treat human and animal ailments. Most people in developing countries use traditional plant-based medicine for basic care due to its affordability, availability, and cultural acceptability. This review aims to gather comprehensive information on plants and their compounds that exhibit anti-gout activities, focusing on in vitro and in vivo investigations. The scientific database was searched by using different keywords, and papers published between March 2008 and December 2023 were considered. The inclusion criteria were publications on inflammatory mediators, free radicals, oxidative stress, or proteins in gout etiology or evolution, along with plant-based remedies. Finally, 27 publications based on inclusion and exclusion criteria reported the active compounds and analyzed parameters and outcomes of research carried out with these medicinal plants. This paper extensively explores the potential mechanism of employing medicinal herbs for the management of gout. However, more investigation is needed to fully comprehend the role that various biomarkers play in the development of GA, as well as to investigate the possible functions of different plants and the substances found in them.

In this study, we utilized high-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (HPLC-Q-TOF/MS/MS), combined with electrospray ionization in the positive mode (ESI+), to establish a comprehensive framework for chromatographic separation and mass spectrometric detection.

The primary focus was on performing collision-induced dissociation (CID) experiments on levantilide D, a newly identified macrolide, and its well-established analog, levantilide C, in order to elucidate their mass spectral fragmentation patterns.

The results revealed that the cleavage patterns of levantilides were successfully characterized when ionized in the positive mode. These fragmentation profiles provide valuable insights for the rapid and accurate structural identification and quantitative analysis of levantilides when they often occur in trace amounts.

Furthermore, this methodology offers crucial technical references for environmental pollutant monitoring and pharmacokinetic investigations for levantilide-related compounds.