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2000
Volume 16, Issue 1
  • ISSN: 2210-3155
  • E-ISSN: 2210-3163

Abstract

Introduction

(Urticaceae) is widespread throughout Brazil and is commonly known as “mapati”. Terpenes are common in Urticaceae species. However, few studies have reported the isolation and characterisation of these compounds in genus.

Methods

The aim of this study was to separate substances from stem barks of using a high-performance countercurrent chromatography gradient elution method. The dichloromethane extract of the stem bark was submitted to silica gel column chromatography, yielding three fractions. Fraction 3 was submitted to a gradient elution system in high-performance countercurrent chromatography using two solvent systems, hexane/ethyl acetate/methanol/water (1:2:x:1, x=1.0 and 1.5). Six compounds were isolated.

Results

A new megastigmane nor-sesquiterpene named 2-acetyl-4,7-epoxy-3-oxy-5,5,9-trimethylhexahydrocoumaran (1) was obtained together with five known terpenes: vomifoliol (2), 2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid (3), 3-β-D-arabinopyranoside-19-hydroxyurs-12-en-28-oic acid (4), arjunolic acid (5), and tormentic acid (6). Fractions 1 and 2 were purified by binary gradient elution from hexane to ethyl acetate.

Conclusion

This process isolated three known compounds: friedelin (7) and 3β-friedelanol (8) from fraction 1, and β-sitosterol (9) from fraction 2. Gradient elution in countercurrent chromatography was a suitable alternative, exploiting variations in the partition coefficient between substances with small structural differences.

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A New Megastigmane Nor-sesquiterpene Isolated from Pourouma guianensis by High-Performance Countercurrent Chromatography
Natural Products Journal, The 16, E22103155332480 (2026); https://doi.org/10.2174/0122103155332480241008110251
/content/journals/npj/10.2174/0122103155332480241008110251
/content/journals/npj/10.2174/0122103155332480241008110251
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  • Article Type:     Research Article
Keyword(s): Phytochemistry; Pourouma guianensis; skin lesions; terpenoids; triterpene; urticaceae

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