Sensitive Detection of Human Hemoglobin by MWCNTs-ionic Liquid: Anthraquinone Modified Electrode

Introduction: Today, nano-composite gels based on multi-walled carbon nanotubes in room temperature ionic liquid (MWCNTs-IL-Gel) are an interesting subject. Materials and Methods: The nano-composite gels showed convenient electrochemical properties against redox activities of electroactive biomolecules. Also, the evaluation of hemoglobin concentration is a critical point in the clinical sample. So, the kind of nano-composite gel which is composed of NH2 functionalized MWCNTs and [amim] Br IL and anthraquinone 2-carboxilic acid (AQ) was fabricated and applied in electrochemical detection of hemoglobin. Cyclic voltammograms of NH2.MWCNTs-IL-AQ modified electrode exhibited redox peak sat -0.5 V (vs. Ag/AgCl) in 0.1 M phosphate buffer solution ((pH 7.0). Results: As the hemoglobin added into the measured solution, the redox current decreased. Conclusion: It can be concluded that the increment in the concentration of hemoglobin caused the decrease in the redox currents in cyclic voltammetry. The NH2.MWCNTs-IL-AQ would detect the concentration of hemoglobin from 1.64 x10−8 to 4.89x10−7 M.