Topical Delivery of Nano-encapsulated Rutoside Medication for Diabetic Foot Ulcer in Rat Model

Introduction: To prepare nano-encapsulated Rutoside ointment (NRO) and studied their topical delivery on diabetic foot ulcer (DFU) rats. Encapsulate the Rutoside with palmitic acid and polyvinyl alcohol using hot homogenization method. Rutoside nanoparticles are characterized with transmission electron microscopy, atomic force microscopy and infrared spectroscopy. NRO develops with absorption ointment ingredients and evaluated their pH, viscosity, and stability. Method: The standardized wound area created on the foot surface of diabetic rats. The foot ulcer healing nature of NRO and Rutoside ointment (RO) determined by wound contraction, histopathological scores, hydroxyl proline and antioxidant levels on the day of 1st, 8th and 16th day. Formulation 5 displayed spherically and rod shape nanoparticles, with excellent encapsulation and stability. NRO presents pH (6.9 ± 03) and viscosity (17.23 ± 0.02). Result: Topical delivery of NRO showed significant wound contraction (p < 0.01), increased antioxidants and hydroxyl proline content than compared with DFU rats treated with RO on the 16th day. Efficient regeneration of dermal tissues, capillary vessels and thickness of granulation tissues also noted in NRO treated rats. Conclusion: The study infers NRO effectively improved wound contraction in the diabetic foot ulcer rats.