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2000
Volume 23, Issue 1
  • ISSN: 1570-1786
  • E-ISSN: 1875-6255

Abstract

Bioethanol production from duckweed () plants is a promising approach to producing renewable energy. Due to its advantageous characteristics, such as being a non-food feedstock, the fastest-growing angiosperm, globally adaptable to various climates, capable of high starch accumulation under nutrient modifications, and possessing low to no lignin content, Lemna minor is a promising candidate for bioethanol production. This research aimed to produce starch-enhanced under conditions of nutritional starvation. The starch enhancement technique was standardized by performing experiments sequentially with organic manure, nitrogen-free Hoagland media, and full-strength Hoagland media. Initially, the starch quantity was found to be 7%. However, it was observed that during nitrogen stress, high starch accumulation occurred in , and on the 9th day, it reached its maximum, at 26% with a standard deviation of ±0.3. It was also observed that the protein, glucose, and fructose composition dropped during this experiment. One-way ANOVA analysis was performed for statistical analysis. It was found that during the starch enhancement experiment, the starch level was significantly (P < 0.05) higher in nitrogen-free Hoagland media compared to the organic manure. Therefore, may be used as a substitute for commercial food feedstocks in bioethanol production.

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References

  1. Fischer-KowalskiM. RovenskayaE. KrausmannF. PalluaI. Mc NeillJ.R. Technol. Forecast. Soc. Change2019138697710.1016/j.techfore.2018.08.010
    [Google Scholar]
  2. NguyenT.V.T. UnpapromY. ManmaiN. WhangchaiK. RamarajR. Biomass Convers. Biorefin.20221251605161710.1007/s13399‑020‑00977‑7
    [Google Scholar]
  3. CherubiniF. StrømmanA.H. Biofuels - Alternative Feedstocks and Conversion Processes.New York, USAAcademic Press201110.1016/B978‑0‑12‑385099‑7.00001‑2
    [Google Scholar]
  4. LangeJ.P. Biofuels Bioprod. Biorefin.200711394810.1002/bbb.7
    [Google Scholar]
  5. NaghshbandiM.P. TabatabaeiM. AghbashloM. GuptaV.K. SulaimanA. KarimiK. MoghimiH. MalekiM. Renew. Sustain. Energy Rev.201911510935310.1016/j.rser.2019.109353
    [Google Scholar]
  6. KhanalS.K. Anaerobic biotechnology for bioenergy production: Principles and applications.Ames, IA, USAWiley-Blackwell200810.1002/9780813804545
    [Google Scholar]
  7. NielsenF. GalbeM. ZacchiG. WallbergO. Biomass Convers. Biorefin.202010225326610.1007/s13399‑019‑00454‑w
    [Google Scholar]
  8. ZabedH. SahuJ.N. SuelyA. BoyceA.N. FaruqG. Renew. Sustain. Energy Rev.20177147550110.1016/j.rser.2016.12.076
    [Google Scholar]
  9. YükselF. YükselB. Renew. Energy20042971181119110.1016/j.renene.2003.11.012
    [Google Scholar]
  10. World Bio Energy. WBA Global Bioenergy Statistics.2017Available from: www.worldbioenergy.org/global-bioenergy-statistics.
  11. Serrano-RuizJ.C. DumesicJ.A. Energy Environ. Sci.201141839910.1039/C0EE00436G
    [Google Scholar]
  12. BridgwaterA.V. Biomass Bioenergy201238689410.1016/j.biombioe.2011.01.048
    [Google Scholar]
  13. ToorS.S. RosendahlL. RudolfA. Energy20113652328234210.1016/j.energy.2011.03.013
    [Google Scholar]
  14. XuJ. CuiW. ChengJ.J. StompA.M. Bioresour. Technol.2011102210551060
    [Google Scholar]
  15. BhaskarT. BhavyaB. SinghR. NaikD.V. KumarA. GoyalH.B. Thermochemical conversion of biomass to biofuels.Oxford, UKAcademic Press20115177
    [Google Scholar]
  16. NaikS.N. GoudV.V. RoutP.K. DalaiA.K. Renew. Sustain. Energy Rev.201014257859710.1016/j.rser.2009.10.003
    [Google Scholar]
  17. AyodeleB.V. AlsaffarM.A. MustapaS.I. J. Clean. Prod.202024511885710.1016/j.jclepro.2019.118857
    [Google Scholar]
  18. DeviA. BajarS. SihagP. SheikhZ.U.D. SinghA. KaurJ. BishnoiN.R. PantD. Bioengineered20231418111210.1080/21655979.2022.2095702 37401849
    [Google Scholar]
  19. ZhangH. ZhangP. WuT. RuanH. Fermentation20239870910.3390/fermentation9080709
    [Google Scholar]
  20. SreeK. BogM. AppenrothK. Emir. J. Food Agric.201628529130210.9755/ejfa.2016‑01‑038
    [Google Scholar]
  21. ChenQ. JinY. ZhangG. FangY. XiaoY. ZhaoH. Energies2012583019303210.3390/en5083019
    [Google Scholar]
  22. SembadaA.A. FaizalA. AIP Conf Proc2019212003002610.1063/1.5115630
    [Google Scholar]
  23. FaizalA. SembadaA.A. PrihartoN. Saudi J. Biol. Sci.202128129430110.1016/j.sjbs.2020.10.002 33424309
    [Google Scholar]
  24. GönenÇ. Sugar Tech201820447448110.1007/s12355‑017‑0558‑y
    [Google Scholar]
  25. KumarA.K. SharmaS. Bioresour. Bioprocess.201741710.1186/s40643‑017‑0137‑9 28163994
    [Google Scholar]
  26. LeeC.J. YangchengH. ChengJ.J. JaneJ.L. Stärke2015683–4348354
    [Google Scholar]
  27. ZhaoX. MoatesG.K. WilsonD.R. GhogareR.J. ColemanM.J. WaldronK.W. Biomass Bioenergy20157220621510.1016/j.biombioe.2014.11.003
    [Google Scholar]
  28. AgborV.B. CicekN. SparlingR. BerlinA. LevinD.B. Biotechnol. Adv.201129667568510.1016/j.biotechadv.2011.05.005 21624451
    [Google Scholar]
  29. ChiaramontiD. PrussiM. FerreroS. OrianiL. OttonelloP. TorreP. CherchiF. Biomass Bioenergy201246253510.1016/j.biombioe.2012.04.020
    [Google Scholar]
  30. TalebniaF. KarakashevD. AngelidakiI. Bioresour. Technol.2010101134744475310.1016/j.biortech.2009.11.080 20031394
    [Google Scholar]
  31. TaherzadehM.J. KarimiK. Int. J. Mol. Sci.2008991621165110.3390/ijms9091621 19325822
    [Google Scholar]
  32. SindhuR. BinodP. PandeyA. Bioresour. Technol.2016199768210.1016/j.biortech.2015.08.030 26320388
    [Google Scholar]
  33. CuiW. XuJ. JayJ. In: American Society of Agricultural and Biological EngineersPittsburgh, Pennsylvania, June 20-June 232010100944010.13031/2013.29907
    [Google Scholar]
  34. MuradovN. FidalgoB. GujarA.C. GarceauN. T-RaissiA. Biomass Bioenergy20124212313110.1016/j.biombioe.2012.03.003
    [Google Scholar]
  35. KuznetsovaT. PolitaevaN. SmyatskayaY. IvanovaA. IOP Conf. Ser. Earth Environ. Sci.2019272202205810.1088/1755‑1315/272/2/022058
    [Google Scholar]
  36. VermaR. SutharS. Energy Sources A Recovery Util. Environ. Effects201638152231223710.1080/15567036.2015.1098750
    [Google Scholar]
  37. ReddyK.R. De BuskW.F. J. Environ. Qual.198514445946210.2134/jeq1985.00472425001400040001x
    [Google Scholar]
  38. CulleyD.D. EppsE.A. J. Water Pollut. Control Fed.1973452337347
    [Google Scholar]
  39. ZhaoY. FangY. JinY. HuangJ. BaoS. FuT. HeZ. WangF. ZhaoH. Bioresour. Technol.2014163829110.1016/j.biortech.2014.04.018 24787320
    [Google Scholar]
  40. IqbalS. Duckweed aquaculture potentials: Possibilities and limitations for combined wastewater treatment and animal feed production in developing countries. EAWAG, SANDEC Report No. 6/99.1999Available from: https://www.feedipedia.org/node/17871.
  41. ZhaoZ. ShiH. WangM. CuiL. ZhaoH. ZhaoY. Plant Physiol. Biochem.201586728110.1016/j.plaphy.2014.11.007 25438139
    [Google Scholar]
  42. ShiH. ErnstE. HeinzelN. McCorkleS. RolletschekH. BorisjukL. OrtlebS. MartienssenR. ShanklinJ. SchwenderJ. BMC Plant Biol.202323145810.1186/s12870‑023‑04480‑9 37789269
    [Google Scholar]
  43. KleczkowskiL.A. VillandP. LönneborgA. OlsenO.A. LüthiE.Z. Naturforsch C J. Biosci.1991467-860561210.1515/znc‑1991‑7‑817 1663749
    [Google Scholar]
  44. WolvertonB.C. McdonaldR.C. Econ. Bot.198135222423210.1007/BF02858689
    [Google Scholar]
  45. CarnovaleG. LeiversS. RosaF. NorliH.R. HortemoE. WicklundT. HornS.J. SkjånesK. Foods20221110144910.3390/foods11101449 35627018
    [Google Scholar]
  46. MeussdoerfferF. ZarnkowM. In: Handbook of Brewing: Processes, Technology, Markets. EßlingerH.M. Wiley200910.1002/9783527623488.ch2
    [Google Scholar]
  47. ZieglerP. AppenrothK.J. SreeK.S. Plants20231211221510.3390/plants12112215 37299193
    [Google Scholar]
  48. AppenrothK.J. KrechK. KeresztesÁ. FischerW. KoloczekH. Plant Biol.2010124686692
    [Google Scholar]
  49. SreeK.S. AdelmannK. GarciaC. LamE. AppenrothK.J. Planta201524161395140410.1007/s00425‑015‑2264‑x 25693515
    [Google Scholar]
  50. LiuY. XuH. WangY. TangX. HeG. WangS. MaY. KongY. YuC. ZhouG. Glob. Change Biol. Bioenergy202012121078109110.1111/gcbb.12746
    [Google Scholar]
  51. HumphreyT.J. SarawekS. DaviesD.D. Planta1977137325926410.1007/BF00388160 24420663
    [Google Scholar]
  52. YuC. ZhaoX. QiG. BaiZ. WangY. WangS. MaY. LiuQ. HuR. ZhouG. Biotechnol. Biofuels201710116710.1186/s13068‑017‑0851‑8 28670341
    [Google Scholar]
  53. KrugerK. ChenL. HeB.B. J. Environ. Qual.20204941044105310.1002/jeq2.20092 33016485
    [Google Scholar]
  54. AppenrothK.J. SreeK.S. BöhmV. HammannS. VetterW. LeitererM. JahreisG. Food Chem.201721726627310.1016/j.foodchem.2016.08.116 27664634
    [Google Scholar]
  55. LandoltE. KandelerR. Geobot Inst1987
    [Google Scholar]
  56. GeisselerD. SmithR. CahnM. MuramotoJ. J. Environ. Qual.20215061325133810.1002/jeq2.20295 34664278
    [Google Scholar]
  57. AdameC.R. CarlosR.S. BraosL.B. FerreiraM.E. da CruzM.C.P. Nitrogen2024519110510.3390/nitrogen5010007
    [Google Scholar]
  58. UllahH. GulB. KhanH. AkhtarN. RehmanK.U. ZebU. BMC Plant Biol.202222121410.1186/s12870‑022‑03600‑1 35468717
    [Google Scholar]
  59. OpiyoM. MbogoK. ObieroK. OrinaS.J. Limnol Freshw.Fish. Res.20239312312910.17216/limnofish.1152512
    [Google Scholar]
  60. AppenrothK.J. KrechK. KeresztesÁ. FischerW. KoloczekH. Chemosphere201078321622310.1016/j.chemosphere.2009.11.007 19945735
    [Google Scholar]
  61. AppenrothK.J. StöckelJ. SrivastavaA. StrasserR.J. Environ. Pollut.20011151496410.1016/S0269‑7491(01)00091‑4 11586773
    [Google Scholar]
  62. SreeK.S. AppenrothK.J. Albanian J. Agric. Sci.2014131114
    [Google Scholar]
  63. HouW. ChenX. SongG. WangQ. Chi ChangC. Plant. Physiol. Biochem.2007451626910.1016/j.plaphy.2006.12.005 17300947
    [Google Scholar]
  64. SreeK.S. KeresztesÁ. Mueller-RoeberB. BrandtR. EberiusM. FischerW. AppenrothK.J. Chemosphere201513114915610.1016/j.chemosphere.2015.03.008 25840119
    [Google Scholar]
  65. ChenG. FangY. HuangJ. ZhaoY. LiQ. LaiF. XuY. TianX. HeK. JinY. TanL. ZhaoH. RSC Advances2018832179271793710.1039/C8RA01856A 35542060
    [Google Scholar]
  66. GuptaH. DhimanS. KumarA. PaliwalS.K. KhanG. SinghH. MishraA. MishraA.K. Separ. Purif. Rev.202554131610.1080/15422119.2024.2332953
    [Google Scholar]
  67. ChormareR. KumarM.A. Chemosphere202230213483610.1016/j.chemosphere.2022.134836 35525441
    [Google Scholar]
  68. Sumate Chaiprapat Jiayang Cheng ClassenJ. LiehrS. Trans. ASAE20024810.13031/2013.10499
    [Google Scholar]
  69. LengR.A. StambolieJ.H. BellR. Livest. Res. Rural Dev.199571
    [Google Scholar]
  70. Van DyckI. VanhoudtN. Vives i BatlleJ. HoremansN. Van GompelA. NautsR. VangronsveldJ. VangronsveldJ. Plant. Physiol. Biochem.202320010775510.1016/j.plaphy.2023.107755 37216822
    [Google Scholar]
  71. TuD.T.M. DongN.T.K. PrestonT.R. Livest. Res. Rural Dev.20122471
    [Google Scholar]
  72. BergmannB.A. ChengJ. ClassenJ. StompA.M. Bioresour. Technol.2000731132010.1016/S0960‑8524(99)00137‑6
    [Google Scholar]
  73. Guidelines for single laboratory validation of chemical methods for dietary supplements and botanicals.2002Available from: https://s27415.pcdn.co/wp-content/uploads/2020/01/64ER20-7/Validation_Methods/d-AOAC_Guidelines_For_Single_Laboratory_Validation_Dietary_Supplements_and_Botanicals.pdf.
  74. MillerG.L. Anal. Chem.195931342642810.1021/ac60147a030
    [Google Scholar]
  75. KjeldahlJ. Fresenius Z.Anal. Chem.188322136638210.1007/BF01338151
    [Google Scholar]
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