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2000
Volume 21, Issue 19
  • ISSN: 1570-1808
  • E-ISSN: 1875-628X

Abstract

Background

Obstructive sleep apnea (OSA) is characterized by recurrent stenosis or collapse of the upper airway during sleep. This directly leads to reduced ventilation or apnea, followed by arterial hypoxemia and hypercapnia.

Aim

The study aims to explore whether hydrogen sulfide (H2S) could serve as a protective factor for the hypoglossal nucleus (HN) against chronic intermittent hypoxia (CIH) in rats.

Methods

A total of 12 adult male Sprague-Dawley (SD) rats were randomly and equally divided into two groups (CIH and control group). Rats in the CIH group were housed in a hypoxic chamber with the oxygen volume fraction alternating between 21% and 5% by providing air for 60 s and nitrogen for 60 s from 8:30am to 16:30pm each day for 35 days. The control group was housed in a chamber with a normal oxygen level. After 5 weeks, expressions of different synthases in the HN were detected using Western blot analysis and qRT-PCR.

Results

Transcriptions of synthetase gene CBS ( < 0.01) and 3MST ( < 0.05) in the CIH group were significantly reduced compared to those in the control group. Expression of H2S synthetase 3MST was significantly down-regulated in the hypoglossal nucleus of CIH rats compared to that in the control group ( < 0.05). The expression of CBS was significantly reduced in the CIH group compared to that in control group ( < 0.05).

Conclusion

CBS and 3MST-H2S pathways may be involved in regulating hypoglossal nerve activity related to respiration and protecting the HN from CIH-induced injury. This study suggests that the CBS and 3MST-H2S pathways could be one of the important pathogenesis of OSA.

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2025-09-01

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Hydrogen Sulfide (H2S) Synthetases in the Hypoglossal Nucleus Involved in Chronic Intermittent Hypoxia Rats: A Pilot Study
Letters in Drug Design & Discovery 21, 4976 (2024); https://doi.org/10.2174/0115701808358454250219114915
/content/journals/lddd/10.2174/0115701808358454250219114915
/content/journals/lddd/10.2174/0115701808358454250219114915
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  • Article Type:     Research Article
Keyword(s): Chronic intermittent hypoxia; chronic intermittent hypoxia; hydrogen sulfide; hydrogen sulfide synthase; hypoglossal nucleus; obstructive sleep apnea

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