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image of Phylogenetic Analysis of SOD Gene Isolated from Indian Variety of Mud Crabs: Scylla serrata and Scylla olivacea

Abstract

Aim

Our research aimed to isolate and sequence the SOD gene from the genomic DNA of and and to study its phylogeny.

Background

In crustaceans, superoxide dismutase (SOD) serves as the first line of defense against stress. Extracellular Cu/Zn-SOD has been demonstrated in several investigations involving crustaceans. Crustaceans do not have a distinct immune system. They entirely depend on the innate immune system triggered when they come in contact with any pathogen.

Methods

Partial SOD gene was isolated from the genomic DNA of and through polymerase chain reaction.

Results

We successfully isolated partial SOD genes of 942bp and 957bp from and , respectively. The sequences were submitted to the NCBI GenBank database.

Discussion

The phylogenetic study suggests their clustering with the genus species. Investigating the SOD gene sequences across diverse crustacean lineages can reveal profound insights into their evolutionary history and the intricate relationships among species concerning their SOD development.

Conclusion

This research holds the potential to enhance our understanding of the evolutionary adaptations that have shaped these organisms.

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2025-06-05
2025-09-23

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Phylogenetic Analysis of SOD Gene Isolated from Indian Variety of Mud Crabs: Scylla serrata and Scylla olivacea
Bentham Science Publishers ; https://doi.org/10.2174/0113892037375925250519112911
/content/journals/cpps/10.2174/0113892037375925250519112911
/content/journals/cpps/10.2174/0113892037375925250519112911
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  • Article Type:     Research Article
Keywords: Mud crabs ; Phylogenetic analysis ; Polymerase chain reaction (PCR) ; Superoxide dismutase (SOD) ; open reading frame (ORF) ; reactive oxygen species (ROS) ; Multiple sequence alignment (MSA)

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