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image of Ni-Fe Nanoparticles from Eugenia jambolana Extract Show Enhanced Anti-Biofilm, Anti-Inflammatory, and Antioxidant Effects

Abstract

Introduction

Metallic nanoparticles are of interest for their potent bactericidal and anti-biofilm effects within a favorable therapeutic index. This study reports the green synthesis of bimetallic nickel-iron (Ni-Fe) nanoparticles using extract and evaluates their antimicrobial, anti-biofilm, anti-inflammatory, and antioxidant activities.

Methods

Ni-Fe nanoparticles were synthesized using extract and characterized for crystalline structure, size, stability, zeta potential, and functional groups. Antimicrobial activity was tested against Gram-positive (, ), Gram-negative (, ), and . Anti-biofilm potential was assessed inhibition and dispersion assays, EPS quantification, and visualization. Anti-inflammatory activity was measured through protein denaturation and nitric oxide scavenging assays, while antioxidant capacity was determined using DPPH and HO scavenging tests.

Results

Crystalline, stable Ni-Fe nanoparticles with favorable functional groups were obtained. At 200 µg/mL, they showed broad-spectrum antimicrobial activity. Biofilm formation was reduced by 50% at 250 µg/mL, and dispersion occurred at 10-50 µg/mL, with most susceptible. EPS inhibition at 50 µg/mL was 78% (), 70% (), 73% (), and 91% (). Visualization confirmed strong adherence to biofilms. At 250 µg/mL, protein denaturation inhibition reached 45%, nitric oxide scavenging 42.6%, DPPH scavenging 44%, and HO scavenging 49%.

Discussion

Ni-Fe nanoparticles exhibit strong antimicrobial, anti-biofilm, anti-inflammatory, and antioxidant activities, notably against . High EPS inhibition and biofilm dispersion suggest potential against biofilm-associated, drug-resistant infections.

Conclusion

Green-synthesized Ni-Fe nanoparticles from show multifunctional bioactivities, offering promise for therapeutic applications targeting resistant and biofilm-related infections.

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2025-10-29
2025-12-19

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Ni-Fe Nanoparticles from Eugenia jambolana Extract Show Enhanced Anti-Biofilm, Anti-Inflammatory, and Antioxidant Effects
Bentham Science Publishers ; https://doi.org/10.2174/0113816128394608251005230040
/content/journals/cpd/10.2174/0113816128394608251005230040
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  • Article Type:     Research Article
Keywords: Eugenia jambolana ; antioxidant ; Biofilm ; anti-inflammatory ; fatty acids ; bimetallic Ni-Fe nanoparticles

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