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2000
Volume 22, Issue 1
  • ISSN: 1573-4137
  • E-ISSN: 1875-6786
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Abstract

Introduction

Stem cell therapy has emerged as a highly active field of research due to the remarkable abilities of stem cells to renew themselves and differentiate into various types of cells when cultured. However, scientists have recently become more aware of the limitations of traditional 2D culture and stem cell culture media.

Methods

This study aims to create an alternative polymeric three-dimensional (3D) scaffold by utilizing the self-assembly process of a star-shaped amphiphilic copolymer (poly(caprolactone) and poly(ethylene oxide)) into nanofibers. These nanofibers closely resemble the native extracellular matrix in terms of scale and capability of replicating the extracellular microenvironment, enabling the observation and manipulation of stem cell functions.

Results

The findings of this study indicate that polymeric nanofibers are highly effective as a 3D scaffold for the proliferation of mouse Embryonic Stem Cells (mESCs) while maintaining their stem cell characteristics.

Conclusion

These findings strongly suggest that the polymeric 3D scaffolds, in the form of nanofibers, not only support the growth and proliferation of stem cells but also preserve the pluripotency of mESCs.

© 2026 The Author(s). Published by Bentham Science Publishers.
This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode
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2025-04-09
2026-01-04

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/content/journals/cnano/10.2174/0115734137341067241231110858
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Nanofibers of Poly(caprolactone)-poly(ethylene oxide) as 3D Scaffolds for Enhancing Stem Cell Growth and Proliferation
Curr. Nanosci. 22, 133 (2026); https://doi.org/10.2174/0115734137341067241231110858
/content/journals/cnano/10.2174/0115734137341067241231110858
/content/journals/cnano/10.2174/0115734137341067241231110858
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  • Article Type:     Research Article
Keyword(s): 3-D culture; extracellular microenvironment; Nanowires; polymer; regenerative medicine; stem cells

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