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2000
Volume 32, Issue 21
  • ISSN: 0929-8673
  • E-ISSN: 1875-533X

Abstract

Background

Commercial Minoxidil (MXD) is commonly used as a vasodilator agent of hair follicles for providing direct dermal papilla cell proliferation and consequently enhancing the rate of hair growth.

Objective

The current study attempted to improve the bioactivity and water solubility of MXD by producing nanocrystal structures and investigating the obtained hair growth-stimulating activity on C57BL/6 mice.

Methods

The MXD nanoparticles (MXD-NPs) were prepared through a bead mill and ultrasonic process and characterized by DLS, XRD, UV-Vis, FTIR, FESEM, TEM, and Zeta-potential techniques.

Results

The cytotoxicity of MXD-NPs was studied on human dermal fibroblast (HDF) by MTT assay. Lastly, we analyzed the comparative hair growth inductive activity of certain MXD-NPs concentrations on C57BL/6 mice. The stabled MXD-NPs (-46 mV, 21.9 nm) caused a significant increase in the hair growth rate of C57BL/6 mice by running a safe site-specific delivery mechanism on the targeted pilosebaceous follicles when compared to MXD.

Conclusion

The MXD-NPs-receiving mice exhibited a greater rate of anagen/telogen follicular when compared with MXD-treated types, which verified the improvement of their hair re-growing and follicular-stimulative activities. Therefore, these outcomes confirmed the potential of MXD-NPs for substituting its commercial solution format as a safe and efficient iso-formulation structure.

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Nanocrystal Structure of Minoxidil: A Safe Stimulator for Hair Growth Factor for C57BL/6 Mice
Curr. Med. Chem. 32, 4343 (2025); https://doi.org/10.2174/0109298673283413240116065844
/content/journals/cmc/10.2174/0109298673283413240116065844
/content/journals/cmc/10.2174/0109298673283413240116065844
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  • Article Type:     Research Article
Keyword(s): C57BL/6 mice; cytotoxicity; hair growth stimulating activity; human dermal fibroblast (HDF); Minoxidil nanoparticles (MXD-NPs); nanocrystal structure

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