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image of Adaptability of Thermotoga Maritima's Glycolysis Pathway in Both Oxic and Anoxic Environments

Abstract

Background

The phylum Thermotogae is composed of five families: Fervidobacteriaceae, Thermatogaceae, Kosmotogaceae, Petrotogaceae, and Mesoaciditogaceae; one class: Thermotogae; and four orders: Kosmotogales, Petrotogales, and Mesoaciditogales. There are thirteen genera in all. The physical and metabolic characteristics of the Thermotogae species reflect the extreme heat from which they were separated. Thermotogae members have a broad spectrum of metabolic capacities, resulting in a pool of valuable chemicals with potential uses in many different sectors.

Method

A 1.5-liter operating capacity bioreactor with a 2.3-liter double-jacket glass volume was utilised to culture in both oxic and anoxic conditions. In addition to temperature, pH, and redox potential, sensors that were installed within the fermentor monitored additional parameters. RNA extraction and cDNA synthesis A total of RNAs was extracted utilising Roche's High Pure RNA reagent. Analysis of glycolysis pathways in was performed by NMR spectroscopy

Result

Based on NMR analysis, our findings demonstrate that uses the EM route to metabolize 90% of glucose in anoxia and the ED pathway for 10%. On the other hand, continues to employ the EM and ED glycolysis routes concurrently when exposed to extended oxidative stress; however, the ED pathway's contribution drops from 10% to around 5%.

Conclusion

Compared to the EM route, the ED pathway has more strongly repressed transcripts that encode its unique enzymes.

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2025-02-06
2025-09-14

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Adaptability of Thermotoga Maritima's Glycolysis Pathway in Both Oxic and Anoxic Environments
Bentham Science Publishers ; https://doi.org/10.2174/0113862073357899250126032044
/content/journals/cchts/10.2174/0113862073357899250126032044
/content/journals/cchts/10.2174/0113862073357899250126032044
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  • Article Type:     Research Article
Keywords: anarobiosis ; oxic ; Tdhermotoga maritima ; embden-meyerhof-parnas (EMP) ; RMN ; entner-doudorof (ED) pathway

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