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Abstract

Objective

Immunoglobulin A (IgA) Nephropathy (IgAN) is characterized by pIgA1 dep-osition in the glomerular mesangium, resulting in podocyte Epithelial-to-Mesenchymal Transi-tion (EMT). Traditional Chinese Medicines (TCMs) have been used for the treatment of IgAN for several years and have demonstrated positive efficacy. The present study aimed to establish a High-Content Screening (HCS) method for identifying wind-dampness dispelling TCM extracts that can mitigate podocyte EMT induced by pIgA1.

Material and Methods

IgA1 from IgAN patients was used to establish a podocyte EMT model. The expression of EMT markers, including desmin, podocalyxin, and podocin, was assessed by Immunocytofluorescence (IF). An image-based HCS method was established to identify wind- dampness dispelling TCMs with the anti-EMT activity of podocyte EMT by automatic acquisi- tion and processing of dual-fluorescent labeled images.

Results

A total of 21 wind-dampness-dispelling TCM extracts were screened using the HCS system, leading to the identification of eight wind-dampness-dispelling TCM extracts exhibiting anti-EMT activity. These eight extracts inhibited the pIgA-induced expression of desmin while upregulating the expression of podocalyxin and podocin.

Conclusion

By quantifying the changes in the expression of EMT markers during pIgA1- induced EMT, this study successfully identified wind-dampness dispelling TCM extracts with anti-EMT properties using an HCS system. In addition, the proposed approach presents a novel avenue for the identification of wind-dampness-dispelling TCMs for the treatment of pIgA1- induced EMT.

© 2025 The Author(s). Published by Bentham Science Publishers.
This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode
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2025-04-29
2025-11-09

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High-Content Screening of Wind-Dampness Dispelling Traditional Chinese Medicines against Podocyte EMT Induced by IgA1
Bentham Science Publishers ; https://doi.org/10.2174/0113862073350741250410053000
/content/journals/cchts/10.2174/0113862073350741250410053000
/content/journals/cchts/10.2174/0113862073350741250410053000
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  • Article Type:     Research Article
Keywords: high-content screening ; drug intervention ; epithelial-to-mesenchymal transition ; Immunoglobulin a nephropathy ; podocyte injury ; wind-dampness dispelling traditional chinese medicines

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