Skip to content
2000
image of Mechanism Study on the Regulation of Th1/Th2 in the Treatment of Idiopathic Membranous Nephropathy by Shengyang Yiwei Decoction

Abstract

Background

Shengyang Yiwei Decoction showed efficacy in idiopathic membranous nephropathy treatment, and this study aimed to assess the underlying molecular mechanisms.

Methods

Rats with passive Heymann nephritis were divided into the model group, the Shengyang Yiwei Decoction group, the JAK2 inhibitor group, and the STAT3 inhibitor group. Healthy rats served as the normal control. 24-hour urinary protein excretion, IgG deposition, renal histopathology, the expression levels of synaptopodin, nephrin, podocalyxin, interferon-γ, interleukin-4, T-box expressed in T cells, GATA binding protein-3, and relevant pathway proteins were measured.

Results

Within the model group, a notable elevation in the 24-h urinary protein level was observed, accompanied by evident IgG deposition, increased glomerular volume, eosinophilic deposits, diminished expression of podocyte marker proteins, and a discernible imbalance in Th1/Th2 cellular immunity. Conversely, in Shengyang Yiwei Decoction and both inhibitor groups, enhancements were observed across the aforementioned indexes.

Conclusion

Shengyang Yiwei Decoction may reduce glomerular podocyte injury through the suppression of the JAK2/STAT3 signaling pathway and modulation of the Th1/Th2 immune balance.

© 2025 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/cchts/10.2174/0113862073322871241108065718
2025-01-13
2025-09-14

  • From This Site

    /content/journals/cchts/10.2174/0113862073322871241108065718
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. Alsharhan L. Beck L.H. Jr Membranous Nephropathy: Core Curriculum 2021. Am. J. Kidney Dis. 2021 77 3 440 453 10.1053/j.ajkd.2020.10.009 33487481
    [Google Scholar]
  2. Lv X Wang J Zhang L Canagliflozin reverses Th1/Th2 imbalance and promotes podocyte autophagy in rats with membranous nephropathy. Front Immunol 2022 13 993869 10.3389/fimmu.2022.99386 36531996
    [Google Scholar]
  3. Hu R Quan S Wang Y Spectrum of biopsy proven renal diseases in Central China: A 10-year retrospective study based on 34,630 cases. Sci Rep. 2020 10 1 10994 10.1038/s41598‑020‑67910‑w 32620914
    [Google Scholar]
  4. Cattran D.C. Brenchley P.E. Membranous nephropathy: Integrating basic science into improved clinical management. Kidney Int. 2017 91 3 566 574 10.1016/j.kint.2016.09.048 28065518
    [Google Scholar]
  5. Li X.Z. Wang X.S. Fan J.W. Yang T.Y. Wang L.J. Sun Y. Yao J.C. Jingfang Mixture regulates balance of spleen T lymphocyte subsets in urticaria mice by inhibiting JAK2-STAT3 signaling pathway. Zhongguo Zhongyao Zazhi 2022 47 20 5473 5480 36471962
    [Google Scholar]
  6. Zhu Y. Su Y. Zhang J. Zhang Y. Li Y. Han Y. Dong X. Li W. Li W. Astragaloside IV alleviates liver injury in type 2 diabetes due to promotion of AMPK/mTOR‑mediated autophagy. Mol. Med. Rep. 2021 23 6 437 10.3892/mmr.2021.12076 33846768
    [Google Scholar]
  7. Li L. Hou X. Xu R. Liu C. Tu M. Research review on the pharmacological effects of astragaloside IV. Fundam. Clin. Pharmacol. 2017 31 1 17 36 10.1111/fcp.12232 27567103
    [Google Scholar]
  8. Gui D. Guo Y. Wang F. Liu W. Chen J. Chen Y. Huang J. Wang N. Astragaloside IV, a novel antioxidant, prevents glucose-induced podocyte apoptosis in vitro and in vivo. PLoS One 2012 7 6 e39824 10.1371/journal.pone.0039824 22745830
    [Google Scholar]
  9. Yao X.M. Liu Y.J. Wang Y.M. Wang H. Zhu B.B. Liang Y.P. Yao W.G. Yu H. Wang N.S. Zhang X.M. Peng W. Astragaloside IV prevents high glucose-induced podocyte apoptosis via downregulation of TRPC6. Mol. Med. Rep. 2016 13 6 5149 5156 10.3892/mmr.2016.5167 27109610
    [Google Scholar]
  10. Guo H. Cao A. Chu S. Wang Y. Zang Y. Mao X. Wang H. Wang Y. Liu C. Zhang X. Peng W. Astragaloside iv attenuates podocyte apoptosis mediated by endoplasmic reticulum stress through upregulating sarco/endoplasmic reticulum Ca2+-ATPase 2 expression in diabetic nephropathy. Front. Pharmacol. 2016 7 500 10.3389/fphar.2016.00500 28066247
    [Google Scholar]
  11. Xing L. Fang J. Zhu B. Wang L. Chen J. Wang Y. Huang J. Wang H. Yao X. Astragaloside IV protects against podocyte apoptosis by inhibiting oxidative stress via activating PPARγ-Klotho-FoxO1 axis in diabetic nephropathy. Life Sci. 2021 269 119068 10.1016/j.lfs.2021.119068 33476631
    [Google Scholar]
  12. Zeng J.H. Wang F. Tan Y. Protective mechanism of Yishenhuashi particle on podocytes in diabetic nephropathy mice. Jilin J. Chin. Med. 2016 04 386 390
    [Google Scholar]
  13. Wang G.H. Yang L.F. Wang S.W. Liu Y.T. Research on the orientated effective components of huangqi in huangqi jianzhong tang against chronic atrophic gastritis based on multi-spectrum–effect correlation. World J. Tradit. Chin. Med. 2024 10 2 244 253 10.4103/wjtcm.wjtcm_48_23
    [Google Scholar]
  14. Wang X. Lang R. Zeng Q. Liang Y. Chen N. Ma Z. Yu R. Jianpi qushi heluo formula alleviates renal damages in passive hemann nephritis in rats by upregulating parkin-mediated mitochondrial autophagy. Sci. Rep. 2021 11 1 18338 10.1038/s41598‑021‑97137‑2 34526554
    [Google Scholar]
  15. Wang X.H. Yang Y.N. Liang Y. Lang R. Zeng Q. Yan L. Yu R.H. Wu C.M. Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula. Biomed. Pharmacother. 2022 145 112475 10.1016/j.biopha.2021.112475 34861636
    [Google Scholar]
  16. Zhang X.X. Gao F. Chen S.Z. Renal protection of yishen tongluo prescription in rats with membranous nephropathy and its influence on AMPK/mTOR/ULK1 signaling pathway. Chin. J. Exper. Tradi. Med. Formul. 2021 27 12 57 66
    [Google Scholar]
  17. Gao Y. Cai C.Y. Wang S.S. Observation on the therapeutic effect of Yishen Huashi granules on nephrotic syndrome patients with idiopathic membranous nephropathy. J. Tradit. Chin. Med. 2020 49 03 33 34
    [Google Scholar]
  18. Ronco P. Debiec H. Antigen identification in membranous nephropathy moves toward targeted monitoring and new therapy. J. Am. Soc. Nephrol. 2010 21 4 564 569 10.1681/ASN.2009121220 20185638
    [Google Scholar]
  19. Nagata M. Podocyte injury and its consequences. Kidney Int. 2016 89 6 1221 1230 10.1016/j.kint.2016.01.012
    [Google Scholar]
  20. Erkan E. Proteinuria and progression of glomerular diseases. Pediatr. Nephrol. 2013 28 7 1049 1058 10.1007/s00467‑012‑2335‑1 23124512
    [Google Scholar]
  21. Shi L. Xiao C. Zhang Y. Xia Y. Zha H. Zhu J. Song Z. Vitamin D/vitamin D receptor/Atg16L1 axis maintains podocyte autophagy and survival in diabetic kidney disease. Ren. Fail. 2022 44 1 694 705 10.1080/0886022X.2022.2063744 35469547
    [Google Scholar]
  22. Lv J.F. Yao Q.L. Hu L.H. The significance of podocyte marker protein podocalyxin in evaluating renal function injury in urine. Guangdong Yixue 2012 33 22 3440 3441
    [Google Scholar]
  23. Ning L. Suleiman H.Y. Miner J.H. Synaptopodin is dispensable for normal podocyte homeostasis but is protective in the context of acute podocyte injury. J. Am. Soc. Nephrol. 2020 31 12 2815 2832 10.1681/ASN.2020050572 32938649
    [Google Scholar]
  24. Zhao Q Dai H Liu X Helper T cells in idiopathic membranous nephropathy. Front Immunol. 2021 12 665629 10.3389/fimmu.2021.665629
    [Google Scholar]
  25. Hertweck A. Vila de Mucha M. Barber P.R. Dagil R. Porter H. Ramos A. Lord G.M. Jenner R.G. The TH1 cell lineage-determining transcription factor T-bet suppresses TH2 gene expression by redistributing GATA3 away from TH2 genes. Nucleic Acids Res. 2022 50 8 4557 4573 10.1093/nar/gkac258 35438764
    [Google Scholar]
  26. Zhang L. Wei W. Anti-inflammatory and immunoregulatory effects of paeoniflorin and total glucosides of paeony. Pharmacol. Ther. 2020 207 107452 10.1016/j.pharmthera.2019.107452 31836457
    [Google Scholar]
  27. Zhao Y. Luan H. Jiang H. Xu Y. Wu X. Zhang Y. Li R. Gegen Qinlian decoction relieved DSS-induced ulcerative colitis in mice by modulating Th17/Treg cell homeostasis via suppressing IL-6/JAK2/STAT3 signaling. Phytomedicine 2021 84 153519 10.1016/j.phymed.2021.153519 33640781
    [Google Scholar]
  28. Zhang Y. Wang S. Dai X. Liu T. Liu Y. Shi H. Yin J. Xu T. Zhang Y. Zhao D. Sukhorukov V. Orekhov A.N. Gao S. Wang L. Zhang D. Simiao San alleviates hyperuricemia and kidney inflammation by inhibiting NLRP3 inflammasome and JAK2/STAT3 signaling in hyperuricemia mice. J. Ethnopharmacol. 2023 312 116530 10.1016/j.jep.2023.116530 37098372
    [Google Scholar]
/content/journals/cchts/10.2174/0113862073322871241108065718
Loading
Mechanism Study on the Regulation of Th1/Th2 in the Treatment of Idiopathic Membranous Nephropathy by Shengyang Yiwei Decoction
Bentham Science Publishers ; https://doi.org/10.2174/0113862073322871241108065718
/content/journals/cchts/10.2174/0113862073322871241108065718
/content/journals/cchts/10.2174/0113862073322871241108065718
Loading

Data & Media loading...


  • Article Type:     Research Article
Keywords: podocytes ; Immune balance ; membranous nephropathy ; passive heymann nephritis rats ; Shengyang Yiwei decoction ; JAK2/STAT3

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test