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2000
Volume 28, Issue 13
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Introduction

Increasing evidence indicates that microRNAs (miRNAs) play a crucial role in modulating tumor growth. This study is centered on investigating the contribution of miR-25 to the progression of Renal Cell Carcinoma (RCC).

Methods

The investigators examined the expression levels of miR-25 and ADAMTS16 in RCC samples and cell lines. The association between miR-25 and ADAMTS16 was validated a luciferase reporter assay. Cell viability, apoptosis, migration, and invasion were evaluated utilizing CCK-8 and flow cytometry techniques, while the expression levels of ADAMTS16,β-catenin, GSK-3β, and p-GSK-3β were assessed through western blot analysis.

Results

The investigation revealed elevated expression levels of miR-25 in RCC tissues. Subsequently, ADAMTS16 was identified as a target of miR-25. Increased miR-25 levels were associated with decreased expression of ADAMTS16, resulting in enhanced cell viability and diminished apoptosis. Conversely, inhibition of miR-25 led to decreased cell viability, proliferation, and migration. Additionally, the researchers observed that miR-25 triggered the phosphorylation of GSK-3β and β-catenin while leaving the total GSK-3β level unaffected.

Conclusion

This study suggests that miR-25 regulates the expression of ADAMTS16 through the Wnt/β-catenin signaling pathway, providing new insights into the cause and potential treatment of RCC.

© 2025 Bentham Science Publishers
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2025-11-01

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References

  1. SiegelR.L. MillerK.D. JemalA. Cancer statistics, 2020.CA Cancer J. Clin.202070173010.3322/caac.21590 31912902
     [Google Scholar]
  2. DenisenkoT.V. BudkevichI.N. ZhivotovskyB. Cell death-based treatment of lung adenocarcinoma.Cell Death Dis.20189211710.1038/s41419‑017‑0063‑y 29371589
     [Google Scholar]
  3. NasimF. SabathB.F. EapenG.A. Lung Cancer.Med. Clin. North Am.2019103346347310.1016/j.mcna.2018.12.006 30955514
     [Google Scholar]
  4. ZappaC. MousaS.A. Non-small cell lung cancer: Current treatment and future advances.Transl. Lung Cancer Res.20165328830010.21037/tlcr.2016.06.07 27413711
     [Google Scholar]
  5. Herrera-SolorioA.M. Peralta-ArrietaI. ArmasL.L. LncRNA SOX2-OT regulates AKT/ERK and SOX2/GLI-1 expression, hinders therapy, and worsens clinical prognosis in malignant lung diseases.Mol. Oncol.202015411101129 33433063
     [Google Scholar]
  6. GoyalB. YadavS.R.M. AwastheeN. GuptaS. KunnumakkaraA.B. GuptaS.C. Diagnostic, prognostic, and therapeutic significance of long non-coding RNA MALAT1 in cancer.Biochim. Biophys. Acta Rev. Cancer20211875218850210.1016/j.bbcan.2021.188502 33428963
     [Google Scholar]
  7. GuyonN. GarnierD. BriandJ. NadaradjaneA. Bougras-CartronG. RaimbourgJ. CamponeM. HeymannD. ValletteF.M. FrenelJ.S. CartronP.F. Anti-PD1 therapy induces lymphocyte-derived exosomal miRNA-4315 release inhibiting Bim-mediated apoptosis of tumor cells.Cell Death Dis.20201112104810.1038/s41419‑020‑03224‑z 33311449
     [Google Scholar]
  8. VuT. YangS. DattaP.K. MiR-216b/Smad3/BCL-2 axis is involved in smoking-mediated drug resistance in non-small cell lung cancer.Cancers2020127187910.3390/cancers12071879 32668597
     [Google Scholar]
  9. HirschbergerS. HinskeL.C. KrethS. MiRNAs: Dynamic regulators of immune cell functions in inflammation and cancer.Cancer Lett.2018431112110.1016/j.canlet.2018.05.020 29800684
     [Google Scholar]
  10. LiQ. WangH. PengH. HuangQ. HuyanT. HuangQ. YangH. ShiJ. MicroRNAs: Key players in bladder cancer.Mol. Diagn. Ther.201923557960110.1007/s40291‑019‑00410‑4 31325035
     [Google Scholar]
  11. BoldriniL. GiordanoM. MelfiF. LucchiM. FontaniniG. Expression of miRNA-25 in young and old lung adenocarcinoma.J. Res. Med. Sci.202126113210.4103/jrms.JRMS_830_19 35126595
     [Google Scholar]
  12. SchwartzC.J. DolgalevI. YoonE. OsmanI. HeguyA. Vega-Saenz de MieraE.C. NimehD. JourG. DarvishianF. Microglandular adenosis is an advanced precursor breast lesion with evidence of molecular progression to matrix-producing metaplastic carcinoma.Hum. Pathol.201985657110.1016/j.humpath.2018.10.021 30428388
     [Google Scholar]
  13. KordowskiF. KolarovaJ. SchafmayerC. BuchS. GoldmannT. MarwitzS. KuglerC. ScheufeleS. GasslingV. NémethC.G. BroschM. HampeJ. LuciusR. RöderC. KalthoffH. SiebertR. AmmerpohlO. ReissK. Aberrant DNA methylation of ADAMTS16 in colorectal and other epithelial cancers.BMC Cancer201818179610.1186/s12885‑018‑4701‑2 30081852
     [Google Scholar]
  14. PyunJ.A. KimS. KwackK. Interaction between thyroglobulin and ADAMTS16 in premature ovarian failure.Clin. Exp. Reprod. Med.201441312012410.5653/cerm.2014.41.3.120 25309856
     [Google Scholar]
  15. WangX. HeY. MackowiakB. GaoB. MicroRNAs as regulators, biomarkers and therapeutic targets in liver diseases.Gut2020704784795 33127832
     [Google Scholar]
  16. WangS. ZhangZ. GaoQ. Transfer of microRNA-25 by colorectal cancer cell-derived extracellular vesicles facilitates colorectal cancer development and metastasis.Mol. Ther. Nucleic Acids20212355256410.1016/j.omtn.2020.11.018 33510943
     [Google Scholar]
  17. ZhangM. WangY. JiangL. SongX. ZhengA. GaoH. WeiM. ZhaoL. LncRNA CBR3-AS1 regulates of breast cancer drug sensitivity as a competing endogenous RNA through the JNK1/MEK4-mediated MAPK signal pathway.J. Exp. Clin. Cancer Res.20214014110.1186/s13046‑021‑01844‑7 33494806
     [Google Scholar]
  18. WangY. TaoB. LiJ. MaoX. HeW. ChenQ. Melatonin inhibits the progression of oral squamous cell carcinoma via inducing miR-25-5p expression by directly targeting NEDD9.Front. Oncol.20201054359110.3389/fonc.2020.543591 33344223
     [Google Scholar]
  19. YuD.H. RuanX.L. HuangJ.Y. LiuX.P. MaH.L. ChenC. HuW.D. LiS. Analysis of the interaction network of Hub miRNAs-Hub genes, being involved in idiopathic pulmonary fibers and its emerging role in non-small cell lung cancer.Front. Genet.20201130210.3389/fgene.2020.00302 32300359
     [Google Scholar]
  20. MeadT.J. ApteS.S. ADAMTS proteins in human disorders.Matrix Biol.201871-7222523910.1016/j.matbio.2018.06.002 29885460
     [Google Scholar]
  21. VermaP. DalalK. ADAMTS-4 and ADAMTS-5: Key enzymes in osteoarthritis.J. Cell. Biochem.2011112123507351410.1002/jcb.23298 21815191
     [Google Scholar]
  22. KelwickR. DesanlisI. WheelerG.N. EdwardsD.R. The ADAMTS (A Disintegrin and Metalloproteinase with Thrombospondin motifs) family.Genome Biol.201516111310.1186/s13059‑015‑0676‑3 26025392
     [Google Scholar]
  23. NandadasaS. FoulcerS. ApteS.S. The multiple, complex roles of versican and its proteolytic turnover by ADAMTS proteases during embryogenesis.Matrix Biol.201435344110.1016/j.matbio.2014.01.005 24444773
     [Google Scholar]
  24. CalS. ObayaA.J. LlamazaresM. GarabayaC. QuesadaV. López-OtínC. Cloning, expression analysis, and structural characterization of seven novel human ADAMTSs, a family of metalloproteinases with disintegrin and thrombospondin-1 domains.Gene20022831-2496210.1016/S0378‑1119(01)00861‑7 11867212
     [Google Scholar]
  25. LindG.E. KleiviK. MelingG.I. TeixeiraM.R. Thiis-EvensenE. RognumT.O. LotheR.A. ADAMTS1, CRABP1, and NR3C1 identified as epigenetically deregulated genes in colorectal tumorigenesis.Cell. Oncol.2006285-6259272 17167179
     [Google Scholar]
  26. de Arao TanI. RicciardelliC. RussellD.L. The metalloproteinase ADAMTS1: A comprehensive review of its role in tumorigenic and metastatic pathways.Int. J. Cancer2013133102263227610.1002/ijc.28127 23444028
     [Google Scholar]
  27. ChenJ. ZhangC. XuX. ZhuX. DaiD. Downregulation of A disintegrin and metallopeptidase with thrombospondin motif type 1 by DNA hypermethylation in human gastric cancer.Mol. Med. Rep.20151222487249410.3892/mmr.2015.3667 25936341
     [Google Scholar]
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ADAMTS16 Suppression by MicroRNA-25 Leads to Oncogenic Properties in Renal Cell Carcinoma
Comb Chem High Throughput Screen 28, 2279 (2025); https://doi.org/10.2174/0113862073321326240718063755
/content/journals/cchts/10.2174/0113862073321326240718063755
/content/journals/cchts/10.2174/0113862073321326240718063755
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  • Article Type:     Research Article
Keyword(s): ADAMTS16; invasion; metastasis; miR-25; proliferation; renal cell carcinoma

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