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2000
Volume 28, Issue 7
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Background

native to the Mediterranean region, belonging to the Lamiaceae family, holds significance in folk medicine. Externally, it is applied for treating cuts and burns, while internally, it is utilized to alleviate inflammation, suppress cough, and address gastrointestinal issues.

Methods

This study aimed to investigate the chemical composition of the essential oil of and to evaluate the antioxidant capacity of the essential oil, as well as the aqueous and ethanolic extracts of the plant. Essential oil analysis was performed using Gas Chromatography-Mass Spectrometry (GC–MS), while 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) radical scavenging activity and inhibition of lipid peroxidation assays were applied to the essential oil, water, and ethanol extracts of the plant.

Results

Spathulenol was found to be the predominant constituent of the essential oil, comprising 25.03% of the oil. Compared to the control group (Propyl gallate for DPPH, IC 0.109; BHT for inhibition of lipid peroxidation, IC 0.133), the essential oil was found to have insignificant antioxidant activity (IC value 10.395 mg/mL for DPPH, 1.051 mg/mL for inhibition of lipid peroxidation). Moreover, ethanolic extract (IC value 1.583 mg/mL for DPPH, 0.029 mg/mL for inhibition of lipid peroxidation) exerted more antioxidant activity than aqueous extract (IC value 1.9017 mg/mL for DPPH, 0.161 mg/mL for inhibition of lipid peroxidation).

Conclusion

Hitherto, this is the earliest report on the composition and activity of the essential oil . However, further investigation of different antioxidant capacity assays is suggested to highlight potential variations in mechanisms of action and subsequent results. Everything considered, this study advances the comprehension of the chemical composition and possible therapeutic uses of , highlighting the need for more research into its uses.

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2025-10-26

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References

  1. SahpazS. SkaltsounisA.L. BailleulF. Polyphenols from Ballota acetabulosa. Biochem. Syst. Ecol.200230660160410.1016/S0305‑1978(01)00117‑X
     [Google Scholar]
  2. PatzakA. Revision der gattung ballota section Ballota. Ann. Naturhist. Mus. Wien1958625786
     [Google Scholar]
  3. DavisP.H. Flora of Turkey and the East Aegean Islands.EdinburghEdinburgh University Press1982
     [Google Scholar]
  4. NewallC.A. AndersonL.A. PhilipsonJ.D. Herbal Medicines, A Guide for Health-care Professionals.London, UKPharmaceutical Press1996
     [Google Scholar]
  5. SeidelV. BailleulF. TillequinF. Terpenoids and phenolics in the genus Ballota L. (Lamiaceae).Recent Res. Dev. Phytochem.199932739
     [Google Scholar]
  6. CouladisM. ChinouI.B. TzakouO. LoukisA. Composition and antimicrobial activity of the essential oil of Ballota pseudodictamnus L. Bentham.Phytother. Res.200216872372610.1002/ptr.104312458473
     [Google Scholar]
  7. Erdem Yeşilada, Gisho Honda, Ekrem Sezik, Mamoru Tabata, Katsumi Goto, Yasumasa Ikeshiro, Traditional medicine in Turkey IV. Folk medicine in the Mediterranean subdivision.J. Ethnopharmacol.1993391313810.1016/0378‑8741(93)90048‑A8331960
     [Google Scholar]
  8. BourinM. Effects of a combined plant extract (Euphytose®) on psychometric performance in healthy volunteers.Psychol. Med.19941414711478
     [Google Scholar]
  9. VuralK. EzerN. ErolK. SaminF.P. Anxiolytic and antidepressant activities of some Ballota species.J. Fac. Pharm. Gazi Univ.1996132932
     [Google Scholar]
  10. ÇitogluG. TankerM. SeverB. EnglertJ. AntonR. AltanlarN. Antibacterial activities of diterpenoids isolated from Ballota saxatilis subsp. saxatilis.Planta Med.199864548448510.1055/s‑2006‑9574949690356
     [Google Scholar]
  11. ÇitoğluG.S. YilmazB.S. TarikahyaB. TipirdamazR. Chemotaxonomy of Ballota species.Chem. Nat. Compd.200541329930210.1007/s10600‑005‑0134‑7
     [Google Scholar]
  12. DülgerB. DülgerG. Antimicrobial activity of the leaves of Ballota acetabulosa on microorganisms isolated from urinary tract infections.Turk. J. Pharm. Sci.20129257262
     [Google Scholar]
  13. MeriçliA.H. MeriçliF. TuzlacıE. Flavonoids of Ballota acetabulosa. Acta Pharmaceutica Turcica198830143144
     [Google Scholar]
  14. RubertoG. BarattaM.T. Antioxidant activity of selected essential oil components in two lipid model systems.Food Chem.200069216717410.1016/S0308‑8146(99)00247‑2
     [Google Scholar]
  15. VukovicN. SukdolakS. SolujicS. NiciforovicN. Antimicrobial activity of the essential oil obtained from roots and chemical composition of the volatile constituents from the roots, stems, and leaves of Ballota nigra from Serbia.J. Med. Food200912243544110.1089/jmf.2008.016419459749
     [Google Scholar]
  16. GioffrèG. UrsinoD. LabateM.L.C. GiuffrèA.M. The peel essential oil composition of bergamot fruit (Citrus bergamia, Risso) of Reggio Calabria (Italy): A review.Emir. J. Food Agric.20203283584510.9755/ejfa.2020.v32.i11.2197
     [Google Scholar]
  17. CasigliaS. BrunoM. ScandoleraE. SenatoreF. SenatoreF. Influence of harvesting time on composition of the essential oil of Thymus capitatus (L.) Hoffmanns. & Link. growing wild in northern Sicily and its activity on microorganisms affecting historical art crafts.Arab. J. Chem.20191282704271210.1016/j.arabjc.2015.05.017
     [Google Scholar]
  18. ChatterjeeS. GuptaS. VariyarP.S. Comparison of essential oils obtained from different extraction techniques as an aid in identifying aroma significant compounds of nutmeg ( Myristicagrans ).Nat. Prod. Commun.20151081934578X150100010.1177/1934578X150100083326434138
     [Google Scholar]
  19. MariaG.A. RiccardoN. Citrus bergamia, Risso: The peel, the juice and the seed oil of the bergamot fruit of Reggio Calabria (South Italy).Emir. J. Food Agric.202032752253210.9755/ejfa.2020.v32.i7.2128
     [Google Scholar]
  20. AdamsR.P. Identification of essential oil components by gas chromatography-quadrupole mass spectroscopy.J Am Soc Mass Spectrom20051611
     [Google Scholar]
  21. BaderA. CaponiC. CioniP.L. FlaminiG. Ivano Morelli, Composition of the essential oil of Ballota undulata, B. nigra ssp. foetida and B. saxatilis.Flavour Fragrance J.200318650250410.1002/ffj.1257
     [Google Scholar]
  22. BishayeeS. BalasubramanianA.S. Lipid peroxide formation in rat brain.J. Neurochem.197118690992010.1111/j.1471‑4159.1971.tb12020.x4398119
     [Google Scholar]
  23. BeugeJ.A. AustS.D. The thiobarbituric acid assay.Methods Enzymol.197852306307
     [Google Scholar]
  24. JambunathanN. Determination and detection of reactive oxygen species (ROS), lipid peroxidation, and electrolyte leakage in plants.Methods Mol. Biol.201063929129710.1007/978‑1‑60761‑702‑0_1820387054
     [Google Scholar]
  25. Brand-WilliamsW. CuvelierM.E. BersetC. Use of a free radical method to evaluate antioxidant activity.Lebensm. Wiss. Technol.1995281253010.1016/S0023‑6438(95)80008‑5
     [Google Scholar]
  26. KayaA. KürkçüoğluM. DinçM. DoğuS. Compositions of the essential oils of Ballota nigra subsp. uncinata and subsp. anatolica from Turkey.Indian J. Pharmaceut. Edu. Res.2017513ss185s18910.5530/ijper.51.3s.9
     [Google Scholar]
  27. BabushokV.I. LinstromP.J. ZenkevichI.G. Retention indices for frequently reported compounds of plant essential oils.J. Phys. Chem. Ref. Data2011404043101, 043101-04314710.1063/1.3653552
     [Google Scholar]
  28. RustaiyanA. MasoudiS. AmeriN. SamieeK. MonfaredA. Volatile constituents of Ballota aucheri Boiss., Stachys benthamiana Boiss. and Perovskia abrotanoides Karel. growing wild in Iran.J. Essent. Oil Res.200618221822110.1080/10412905.2006.9699070
     [Google Scholar]
  29. RiganoD. MarrelliM. FormisanoC. MenichiniF. SenatoreF. BrunoM. ConfortiF. Phytochemical profile of three Ballota species essential oils and evaluation of the effects on human cancer cells.Nat. Prod. Res.201731443644410.1080/14786419.2016.118572227189840
     [Google Scholar]
  30. MajdiM. DastanD. MaroofiH. Chemical composition and antimicrobial activity of essential oils of Ballota nigra subsp. kurdica from Iran.Jundishapur J. Nat. Pharm. Prod.201712e36314
     [Google Scholar]
  31. AbdelshafeekK.A. ElgattarA.A. ZarkoonA.H. AlwahashM.A. ShahatA.A. Investigation of the volatile oils, lipid constituents and biological activity of Ballota andreuzziana, Teucrium zanonii and Verbena tenuisecta in Libya.Asian Pac. J. Trop. Med.20103859460110.1016/S1995‑7645(10)60145‑9
     [Google Scholar]
  32. ErdoğanE.A. EverestA. SönmezÖ. Antimicrobial activity of the essential oil of Ballota saxatilis subsp. brachyodonta (Boiss) P.H. Davis & Doroszenko from Turkey.J. Essent. Oil-Bear. Plants20141761100110410.1080/0972060X.2014.935023
     [Google Scholar]
  33. KılıçÖ. KöktenK. Essential oil and fatty acid compounds of some endemic plant species from Turkey.J. Istanbul. Sabahattin Zaim Univ. Inst. Sci. Technol.202023840
     [Google Scholar]
  34. ĐorđevićA.S. JovanovićO.P. ZlatkovićB.K. StojanovićG.S. Chemical composition of Ballota macedonica VANDAS and Ballota nigra L. ssp. foetida (VIS.) HAYEK essential oils – The chemotaxonomic approach.Chem. Biodivers.201613678278810.1002/cbdv.20150025427144646
     [Google Scholar]
  35. RiccobonoL. Ben JemiaM. SenatoreF. BrunoM. Chemical composition and biological activity of the essential oil of Ballota hispanica (L.) Benth. growing wild in Sicily.Plant Biosyst.201615051065107110.1080/11263504.2014.1001462
     [Google Scholar]
  36. FraternaleD. RicciD. Essential oil composition and antifungal activity of aerial parts of Ballota nigra ssp. foetida collected at flowering and fruiting times.Nat. Prod. Commun.2014971934578X140090010.1177/1934578X140090073325230517
     [Google Scholar]
  37. KazemizadehZ. AminiT. NazariF. HabibiZ. Volatile constituents of Ballota nigra subsp. anatolica from Iran.Chem. Nat. Compd.200945573773810.1007/s10600‑009‑9415‑x
     [Google Scholar]
  38. ErtaşA. BoğaM. YeşilY. Phytochemical profile and ABTS cation radical scavenging, cupric reducing antioxidant capacity and anticholinesterase activities of endemic Ballota nigra L. subsp. anatolica P.H. Davis from Turkey.J. Coast. Life Med.20142555559
     [Google Scholar]
  39. SarikayaA.G. Volatile component composition of Ballota nigra subsp. anatolica at different vegetation periods in Çamlıca province of Kütahya city, Turkey.Appl. Ecol. Environ. Res.20171541929193310.15666/aeer/1504_19291933
     [Google Scholar]
  40. BaşerK.H.C. Essential oils of Labiatae from Turkey resent results.Lamiales Newsletter19943611
     [Google Scholar]
  41. Morteza-SemnaniK. SaeediM. AkbarzadehM. The essential oil composition of Ballota nigra. Chem. Nat. Compd.200743672272310.1007/s10600‑007‑0245‑4
     [Google Scholar]
  42. FraternaleD. BucchiniA. GiamperiL. RicciD. Essential oil composition and antimicrobial activity of Ballota nigra L. ssp foetida.Nat. Prod. Commun.2009441934578X090040010.1177/1934578X090040042919476011
     [Google Scholar]
  43. JamzadM. RustaiyanA. JamzadZ. MasoudiS. Essential oil composition of Salvia indica L., Thymus caucasicus wind. Ex ronniger subsp. grossheimii (ronniger) jalas. and Ballota nigra L. Three Labiatae species from Iran.J. Essent. Oil-Bear. Plants2011141768310.1080/0972060X.2011.10643903
     [Google Scholar]
  44. KolisnykY.S. KovalevaA.M. GoryachaO.V. The study of the volatile oils composition obtained from vegetative and generative organs of Ballota nigra l.Vìsnik farmacìï201402(78)596210.24959/nphj.14.1967
     [Google Scholar]
  45. ChemsaA.E. ZellaguiA. ÖztürkM. ErolE. CeylanO. DuruM.E. GherrafN. Antibiofilm formation, antioxidant and anticholinesterase activities of essential oil and methanol extract of Marrubium deserti de Noé.J. Mater. Environ. Sci.201879931000
     [Google Scholar]
  46. Awadh AliN.A. AlhamzyE.H. ChhetriB.K. DosokyN.S. SetzerW.N. Chemical composition, antimicrobial, and cytotoxic activities of the essential oil of Otostegia fruticosa subsp. schimperi from Yemen.Nat. Prod. Commun.201712969972
     [Google Scholar]
  47. CouladisM. TzakouO. VerykokidouE. HarvalaC. Screening of some Greek aromatic plants for antioxidant activity.Phytother. Res.200317219419510.1002/ptr.126112601688
     [Google Scholar]
  48. CitoğluG.S. ÇobanT. SeverB. İşcanM. Antioxidant properties of Ballota species growing in Turkey.J. Ethnopharmacol.2004922-327528010.1016/j.jep.2004.03.01215138012
     [Google Scholar]
  49. DumluM.U. BulutG.E. Some biological activity investigations on Ballota acetabulosa. J. Pharm. Istanbul Univ.2007395356
     [Google Scholar]
  50. Erdoğan-OrhanI. Sever-YılmazB. AltunM.L. SaltanG. Radical quenching activity, ferric-reducing antioxidant power, and ferrous ion-chelating capacity of 16 Ballota species and their total phenol and flavonoid contents.J. Med. Food20101361537154310.1089/jmf.2009.023721091260
     [Google Scholar]
  51. Ben HsounaA. Ben HalimaN. SmaouiS. HamdiN. Citrus lemon essential oil: Chemical composition, antioxidant and antimicrobial activities with its preservative effect against Listeria monocytogenes inoculated in minced beef meat.Lipids Health Dis.201716114610.1186/s12944‑017‑0487‑528774297
     [Google Scholar]
  52. SavonaG. PiozziF. HansonJ.R. SivernsM. 18-Hydroxyballonigrin, a new diterpenoid from Ballota acetobulosa.J. Chem. Soc., Perkin Trans. 11978110101271127210.1039/p19780001271
     [Google Scholar]
  53. JiménezC. RigueraR. Phenylethanoid glycosides in plants: Structure and biological activity.Nat. Prod. Rep.199411659160610.1039/NP994110059115209134
     [Google Scholar]
  54. YılmazB.S. ÇitoğluG.S. High performance liquid chromatographic analysis of some diterpenoids of the Ballota species.FABAD J. Pharm. Sci.2003281317
     [Google Scholar]
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Ballota acetabulosa (L.) Bentham: A First-Time GC-MS Analysis and Antioxidant Potential of its Essential Oil
Comb Chem High Throughput Screen 28, 1145 (2025); https://doi.org/10.2174/0113862073319327240614113859
/content/journals/cchts/10.2174/0113862073319327240614113859
/content/journals/cchts/10.2174/0113862073319327240614113859
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  • Article Type:     Research Article
Keyword(s): antioxidant activity; Ballota acetabulosa; DPPH; essential oil; GC-MS; spathulenol

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