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2000
Volume 28, Issue 8
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Background

Osteoarthritis (OA) is a chronic progressive joint ailment that is largely predominant worldwide. However, it typically gets worse over time, occurs more frequently, and becomes more crippling.

Objectives

Syringic acid (SA) is a well-known phenolic compound reported to suppress inflammation, cell proliferation, and apoptosis of various cancer cells. Since the role of SA in OA remains unknown, there is a need to hypothesize the anti-inflammatory activities of SA on IL-1β-induced ATDC5 chondrocyte-like cells and to elucidate its protective action against OA.

Methods

The cytotoxicity, inflammatory mediators, mRNA expression of MMPs, ADAMTS, COX-2, and Akt/NF-κB protein expression of SA activity on ATDC5 cells were examined through CCK-8 assay, ELISA, RT-qPCR, and western blot. It was found that SA (10, 20, and 30 µM) did not show any inhibitory effects on the viability of the ATDC5 cells in a concentration-dependent manner.

Results

SA markedly reduced the inflammatory mediators, cytokines, PGE2, MMPs, COX-2, and ADAMTS in a concentration-dependent manner. Likewise, SA expressively attenuated IL-1β-stimulated Akt phosphorylation and NF-κB activation in IL-1β- induced ATDC5 chondrocytes.

Conclusion

This study revealed that SA is a novel candidate applicable for the treatment of OA.

© 2025 Bentham Science Publishers
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2025-09-16

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Syringic Acid Attenuates the IL-1β-Induced Akt Pathway in Chondrocyte ATDC5 Cells
Comb Chem High Throughput Screen 28, 1363 (2025); https://doi.org/10.2174/0113862073286384240227053954
/content/journals/cchts/10.2174/0113862073286384240227053954
/content/journals/cchts/10.2174/0113862073286384240227053954
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  • Article Type:     Research Article
Keyword(s): Akt/NF-κB; ATDC5 cells; MMPs; osteoarthritis; Syringic acid; treatment of OA

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