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2000
Volume 28, Issue 2
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Objective

Lymphoma is the most common malignancy of the haematological system. Jeduxiaoliu formula (JDXLF) exerts good therapeutic effects against lymphoma, however, the mechanisms underlying these effects remain unclear. Therefore, this study aimed to investigate the mechanism of action of JDXLF.

Methods

RNA-Seq was performed to examine the molecular mechanisms underlying the therapeutic effects of JDXLF against lymphoma. CCK-8 assay was performed to examine the effects of JDXLF on the proliferation of lymphoma cells. Electron microscopy was performed to examine the morphology of lymphoma cells. Flow cytometry was performed to examine the apoptosis and cell cycle of lymphoma cells. qPCR and Western blotting were performed to detect the expression of apoptotic genes and proteins. , the tumour-suppressive effect of JDXLF on lymphoma transplanted tumours was examined by establishing a subcutaneous transplantation tumour model in nude mice, and the expression of apoptotic proteins in tumour tissues was analysed immunohistochemical staining.

Results

RNA-Seq revealed 71, 350 and 620 differentially expressed genes (DEGs) in the 1 mg/mL, 4 mg/mL and 8 mg/mL JDXLF treatment groups, respectively. KEGG pathway analysis showed that the DEGs were significantly associated with apoptosis, TNF signalling and NF-κB signalling. experiments revealed that JDXLF inhibited the proliferation of lymphoma (Raji and Jeko-1) cells in a dose-dependent manner, induced apoptosis and upregulated the expression of Bax/Bcl2 and caspase3. experiments revealed that JDXLF had a significant tumour-shrinking effect on mice and increased the expression of the apoptosis-related proteins caspase3 and Bax/Bcl2.

Conclusions

This study indicates that JDXLF can induce apoptosis in lymphoma cells and . We suggest this may provide a direction for further research into lymphoma therapy.

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2025-09-19

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References

  1. JamilA. MukkamallaS.K.R. Lymphoma. StatPearls.Treasure Island, FLStatPearls Publishing2022
     [Google Scholar]
  2. BrayF. FerlayJ. SoerjomataramI. SiegelR.L. TorreL.A. JemalA. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries.CA Cancer J. Clin.201868639442410.3322/caac.21492 30207593
     [Google Scholar]
  3. SungH. FerlayJ. SiegelR.L. LaversanneM. SoerjomataramI. JemalA. BrayF. Global Cancer Statistics 2020: GLOBOCAN Estimates of incidence and mortality worldwide for 36 cancers in 185 countries.CA Cancer J. Clin.202171320924910.3322/caac.21660 33538338
     [Google Scholar]
  4. NaghaviM. AbajobirA.A. AbbafatiC. AbbasK.M. Abd-AllahF. AberaS.F. AboyansV. AdetokunbohO. AfshinA. AgrawalA. AhmadiA. AhmedM.B. AichourA.N. AichourM.T.E. AichourI. AiyarS. AlahdabF. Al-AlyZ. AlamK. AlamN. AlamT. AleneK.A. Al-EyadhyA. AliS.D. Alizadeh-NavaeiR. AlkaabiJ.M. AlkerwiA. AllaF. AllebeckP. AllenC. Al-RaddadiR. AlsharifU. AltirkawiK.A. Alvis-GuzmanN. AmareA.T. AminiE. AmmarW. AmoakoY.A. AnberN. AndersenH.H. AndreiC.L. AndroudiS. AnsariH. AntonioC.A.T. AnwariP. ÄrnlövJ. AroraM. ArtamanA. AryalK.K. AsayeshH. AsgedomS.W. AteyT.M. Avila-BurgosL. AvokpahoE.F.G. AwasthiA. BabalolaT.K. BachaU. BalakrishnanK. BaracA. BarbozaM.A. Barker-ColloS.L. BarqueraS. BarregardL. BarreroL.H. BauneB.T. BediN. BeghiE. BéjotY. BekeleB.B. BellM.L. BennettJ.R. BensenorI.M. BerhaneA. BernabéE. BetsuB.D. BeuranM. BhattS. BiadgilignS. BienhoffK. BikbovB. BisanzioD. BourneR.R.A. BreitbordeN.J.K. BultoL.N.B. BumgarnerB.R. ButtZ.A. Cahuana-HurtadoL. CameronE. CampuzanoJ.C. CarJ. CárdenasR. CarreroJ.J. CarterA. CaseyD.C. Castañeda-OrjuelaC.A. Catalá-LópezF. CharlsonF.J. ChibuezeC.E. Chimed-OchirO. ChisumpaV.H. ChitheerA.A. ChristopherD.J. CiobanuL.G. CirilloM. CohenA.J. ColombaraD. CooperC. CowieB.C. CriquiM.H. DandonaL. DandonaR. DarganP.I. das Neves, J.; Davitoiu, D.V.; Davletov, K.; de Courten, B.; Defo, B.K.; Degenhardt, L.; Deiparine, S.; Deribe, K.; Deribew, A.; Dey, S.; Dicker, D.; Ding, E.L.; Djalalinia, S.; Do, H.P.; Doku, D.T.; Douwes-Schultz, D.; Driscoll, T.R.; Dubey, M.; Duncan, B.B.; Echko, M.; El-Khatib, Z.Z.; Ellingsen, C.L.; Enayati, A.; Ermakov, S.P.; Erskine, H.E.; Eskandarieh, S.; Esteghamati, A.; Estep, K.; Farinha, C.S.S.; Faro, A.; Farzadfar, F.; Feigin, V.L.; Fereshtehnejad, S-M.; Fernandes, J.C.; Ferrari, A.J.; Feyissa, T.R.; Filip, I.; Finegold, S.; Fischer, F.; Fitzmaurice, C.; Flaxman, A.D.; Foigt, N.; Frank, T.; Fraser, M.; Fullman, N.; Fürst, T.; Furtado, J.M.; Gakidou, E.; Garcia-Basteiro, A.L.; Gebre, T.; Gebregergs, G.B.; Gebrehiwot, T.T.; Gebremichael, D.Y.; Geleijnse, J.M.; Genova-Maleras, R.; Gesesew, H.A.; Gething, P.W.; Gillum, R.F.; Giref, A.Z.; Giroud, M.; Giussani, G.; Godwin, W.W.; Gold, A.L.; Goldberg, E.M.; Gona, P.N.; Gopalani, S.V.; Gouda, H.N.; Goulart, A.C.; Griswold, M.; Gupta, R.; Gupta, T.; Gupta, V.; Gupta, P.C.; Haagsma, J.A.; Hafezi-Nejad, N.; Hailu, A.D.; Hailu, G.B.; Hamadeh, R.R.; Hambisa, M.T.; Hamidi, S.; Hammami, M.; Hancock, J.; Handal, A.J.; Hankey, G.J.; Hao, Y.; Harb, H.L.; Hareri, H.A.; Hassanvand, M.S.; Havmoeller, R.; Hay, S.I.; He, F.; Hedayati, M.T.; Henry, N.J.; Heredia-Pi, I.B.; Herteliu, C.; Hoek, H.W.; Horino, M.; Horita, N.; Hosgood, H.D.; Hostiuc, S.; Hotez, P.J.; Hoy, D.G.; Huynh, C.; Iburg, K.M.; Ikeda, C.; Ileanu, B.V.; Irenso, A.A.; Irvine, C.M.S.; Islam, S.M.S.; Jacobsen, K.H.; Jahanmehr, N.; Jakovljevic, M.B.; Javanbakht, M.; Jayaraman, S.P.; Jeemon, P.; Jha, V.; John, D.; Johnson, C.O.; Johnson, S.C.; Jonas, J.B.; Jürisson, M.; Kabir, Z.; Kadel, R.; Kahsay, A.; Kamal, R.; Karch, A.; Karimi, S.M.; Karimkhani, C.; Kasaeian, A.; Kassaw, N.A.; Kassebaum, N.J.; Katikireddi, S.V.; Kawakami, N.; Keiyoro, P.N.; Kemmer, L.; Kesavachandran, C.N.; Khader, Y.S.; Khan, E.A.; Khang, Y-H.; Khoja, A.T.A.; Khosravi, M.H.; Khosravi, A.; Khubchandani, J.; Kiadaliri, A.A.; Kieling, C.; Kievlan, D.; Kim, Y.J.; Kim, D.; Kimokoti, R.W.; Kinfu, Y.; Kissoon, N.; Kivimaki, M.; Knudsen, A.K.; Kopec, J.A.; Kosen, S.; Koul, P.A.; Koyanagi, A.; Kulikoff, X.R.; Kumar, G.A.; Kumar, P.; Kutz, M.; Kyu, H.H.; Lal, D.K.; Lalloo, R.; Lambert, T.L.N.; Lan, Q.; Lansingh, V.C.; Larsson, A.; Lee, P.H.; Leigh, J.; Leung, J.; Levi, M.; Li, Y.; Li Kappe, D.; Liang, X.; Liben, M.L.; Lim, S.S.; Liu, P.Y.; Liu, A.; Liu, Y.; Lodha, R.; Logroscino, G.; Lorkowski, S.; Lotufo, P.A.; Lozano, R.; Lucas, T.C.D.; Ma, S.; Macarayan, E.R.K.; Maddison, E.R.; Magdy Abd El Razek, M.; Majdan, M.; Majdzadeh, R.; Majeed, A.; Malekzadeh, R.; Malhotra, R.; Malta, D.C.; Manguerra, H.; Manyazewal, T.; Mapoma, C.C.; Marczak, L.B.; Markos, D.; Martinez-Raga, J.; Martins-Melo, F.R.; Martopullo, I.; McAlinden, C.; McGaughey, M.; McGrath, J.J.; Mehata, S.; Meier, T.; Meles, K.G.; Memiah, P.; Memish, Z.A.; Mengesha, M.M.; Mengistu, D.T.; Menota, B.G.; Mensah, G.A.; Meretoja, T.J.; Meretoja, A.; Millear, A.; Miller, T.R.; Minnig, S.; Mirarefin, M.; Mirrakhimov, E.M.; Misganaw, A.; Mishra, S.R.; Mohamed, I.A.; Mohammad, K.A.; Mohammadi, A.; Mohammed, S.; Mokdad, A.H.; Mola, G.L.D.; Mollenkopf, S.K.; Molokhia, M.; Monasta, L.; Montañez, J.C.; Montico, M.; Mooney, M.D.; Moradi-Lakeh, M.; Moraga, P.; Morawska, L.; Morozoff, C.; Morrison, S.D.; Mountjoy-Venning, C.; Mruts, K.B.; Muller, K.; Murthy, G.V.S.; Musa, K.I.; Nachega, J.B.; Naheed, A.; Naldi, L.; Nangia, V.; Nascimento, B.R.; Nasher, J.T.; Natarajan, G.; Negoi, I.; Ngunjiri, J.W.; Nguyen, C.T.; Nguyen, Q.L.; Nguyen, T.H.; Nguyen, G.; Nguyen, M.; Nichols, E.; Ningrum, D.N.A.; Nong, V.M.; Noubiap, J.J.N.; Ogbo, F.A.; Oh, I-H.; Okoro, A.; Olagunju, A.T.; Olsen, H.E.; Olusanya, B.O.; Olusanya, J.O.; Ong, K.; Opio, J.N.; Oren, E.; Ortiz, A.; Osman, M.; Ota, E.; Pa, M.; Pacella, R.E.; Pakhale, S.; Pana, A.; Panda, B.K.; Panda-Jonas, S.; Papachristou, C.; Park, E-K.; Patten, S.B.; Patton, G.C.; Paudel, D.; Paulson, K.; Pereira, D.M.; Perez-Ruiz, F.; Perico, N.; Pervaiz, A.; Petzold, M.; Phillips, M.R.; Pigott, D.M.; Pinho, C.; Plass, D.; Pletcher, M.A.; Polinder, S.; Postma, M.J.; Pourmalek, F.; Purcell, C.; Qorbani, M.; Quintanilla, B.P.A.; Radfar, A.; Rafay, A.; Rahimi-Movaghar, V.; Rahman, M.H.U.; Rahman, M.; Rai, R.K.; Ranabhat, C.L.; Rankin, Z.; Rao, P.C.; Rath, G.K.; Rawaf, S.; Ray, S.E.; Rehm, J.; Reiner, R.C.; Reitsma, M.B.; Remuzzi, G.; Rezaei, S.; Rezai, M.S.; Rokni, M.B.; Ronfani, L.; Roshandel, G.; Roth, G.A.; Rothenbacher, D.; Ruhago, G.M.; Sa, R.; Saadat, S.; Sachdev, P.S.; Sadat, N.; Safdarian, M.; Safi, S.; Safiri, S.; Sagar, R.; Sahathevan, R.; Salama, J.; Salamati, P.; Salomon, J.A.; Samy, A.M.; Sanabria, J.R.; Sanchez-Niño, M.D.; Santomauro, D.; Santos, I.S.; Santric Milicevic, M.M.; Sartorius, B.; Satpathy, M.; Schmidt, M.I.; Schneider, I.J.C.; Schulhofer-Wohl, S.; Schutte, A.E.; Schwebel, D.C.; Schwendicke, F.; Sepanlou, S.G.; Servan-Mori, E.E.; Shackelford, K.A.; Shahraz, S.; Shaikh, M.A.; Shamsipour, M.; Shamsizadeh, M.; Sharma, J.; Sharma, R.; She, J.; Sheikhbahaei, S.; Shey, M.; Shi, P.; Shields, C.; Shigematsu, M.; Shiri, R.; Shirude, S.; Shiue, I.; Shoman, H.; Shrime, M.G.; Sigfusdottir, I.D.; Silpakit, N.; Silva, J.P.; Singh, J.A.; Singh, A.; Skiadaresi, E.; Sligar, A.; Smith, D.L.; Smith, A.; Smith, M.; Sobaih, B.H.A.; Soneji, S.; Sorensen, R.J.D.; Soriano, J.B.; Sreeramareddy, C.T.; Srinivasan, V.; Stanaway, J.D.; Stathopoulou, V.; Steel, N.; Stein, D.J.; Steiner, C.; Steinke, S.; Stokes, M.A.; Strong, M.; Strub, B.; Subart, M.; Sufiyan, M.B.; Sunguya, B.F.; Sur, P.J.; Swaminathan, S.; Sykes, B.L.; Tabarés-Seisdedos, R.; Tadakamadla, S.K.; Takahashi, K.; Takala, J.S.; Talongwa, R.T.; Tarawneh, M.R.; Tavakkoli, M.; Taveira, N.; Tegegne, T.K.; Tehrani-Banihashemi, A.; Temsah, M-H.; Terkawi, A.S.; Thakur, J.S.; Thamsuwan, O.; Thankappan, K.R.; Thomas, K.E.; Thompson, A.H.; Thomson, A.J.; Thrift, A.G.; Tobe-Gai, R.; Topor-Madry, R.; Torre, A.; Tortajada, M.; Towbin, J.A.; Tran, B.X.; Troeger, C.; Truelsen, T.; Tsoi, D.; Tuzcu, E.M.; Tyrovolas, S.; Ukwaja, K.N.; Undurraga, E.A.; Updike, R.; Uthman, O.A.; Uzochukwu, B.S.C.; van Boven, J.F.M.; Vasankari, T.; Venketasubramanian, N.; Violante, F.S.; Vlassov, V.V.; Vollset, S.E.; Vos, T.; Wakayo, T.; Wallin, M.T.; Wang, Y-P.; Weiderpass, E.; Weintraub, R.G.; Weiss, D.J.; Werdecker, A.; Westerman, R.; Whetter, B.; Whiteford, H.A.; Wijeratne, T.; Wiysonge, C.S.; Woldeyes, B.G.; Wolfe, C.D.A.; Woodbrook, R.; Workicho, A.; Xavier, D.; Xiao, Q.; Xu, G.; Yaghoubi, M.; Yakob, B.; Yano, Y.; Yaseri, M.; Yimam, H.H.; Yonemoto, N.; Yoon, S-J.; Yotebieng, M.; Younis, M.Z.; Zaidi, Z.; Zaki, M.E.S.; Zegeye, E.A.; Zenebe, Z.M.; Zerfu, T.A.; Zhang, A.L.; Zhang, X.; Zipkin, B.; Zodpey, S.; Lopez, A.D.; Murray, C.J.L. Global, regional, and national age-sex specific mortality for 264 causes of death, 1980–2016: A systematic analysis for the Global Burden of Disease Study 2016.Lancet2017390101001151121010.1016/S0140‑6736(17)32152‑9 28919116
     [Google Scholar]
  5. CaoW. ChenH.D. YuY.W. LiN. ChenW.Q. Changing profiles of cancer burden worldwide and in China: A secondary analysis of the global cancer statistics 2020.Chin. Med. J.2021134778379110.1097/CM9.0000000000001474 33734139
     [Google Scholar]
  6. WangL. QinW. HuoY.J. LiX. ShiQ. RaskoJ.E.J. JaninA. ZhaoW.L. Advances in targeted therapy for malignant lymphoma.Signal Transduct. Target. Ther.2020511510.1038/s41392‑020‑0113‑2 32296035
     [Google Scholar]
  7. RoschewskiM. StaudtL.M. WilsonW.H. Diffuse large B-cell lymphoma—treatment approaches in the molecular era.Nat. Rev. Clin. Oncol.2014111122310.1038/nrclinonc.2013.197 24217204
     [Google Scholar]
  8. FengX. HuQ. Advances in the study of molecules of lymphoma-related signaling pathways regulated by Chinese medicine monomer and compound.J. Med. Res.2021092326
     [Google Scholar]
  9. WangY. ZhangQ. ChenY. LiangC.L. LiuH. QiuF. DaiZ. Antitumor effects of immunity-enhancing traditional Chinese medicine.Biomed. Pharmacother.202012110957010.1016/j.biopha.2019.109570 31710893
     [Google Scholar]
  10. Hu, Min Retrospective cohort study of Jeduxiaoliu formula to improve progression-free survival in patients with B-cell non-Hodgkin’s lymphoma.Modern Chin. Med. Clin.2023013137
     [Google Scholar]
  11. HuM. Clinical study of Jeduxiaoliu formula combined with thalidomide tablets in the treatment of elderly or refractory relapsed lymphoma.Chin. J. Trad. Chin. Med. Inf.20230316
     [Google Scholar]
  12. RuJ. LiP. WangJ. ZhouW. LiB. HuangC. LiP. GuoZ. TaoW. YangY. XuX. LiY. WangY. YangL. TCMSP: A database of systems pharmacology for drug discovery from herbal medicines.J. Cheminform.2014611310.1186/1758‑2946‑6‑13 24735618
     [Google Scholar]
  13. JaffeE.S. Diagnosis and classification of lymphoma: Impact of technical advances.Semin. Hematol.2019561303610.1053/j.seminhematol.2018.05.007 30573042
     [Google Scholar]
  14. ThammD.H. Novel treatments for lymphoma.Vet. Clin. North Am. Small Anim. Pract.201949590391510.1016/j.cvsm.2019.04.004 31153610
     [Google Scholar]
  15. ChipukJ.E. MoldoveanuT. LlambiF. ParsonsM.J. GreenD.R. The BCL-2 family reunion.Mol. Cell201037329931010.1016/j.molcel.2010.01.025 20159550
     [Google Scholar]
  16. KrajewskiS. TanakaS. TakayamaS. SchiblerM.J. FentonW. Investigation of the subcellular distribution of the Bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes.Cancer Res.1993531947014714
     [Google Scholar]
  17. OlaM.S. NawazM. AhsanH. Role of Bcl-2 family proteins and caspases in the regulation of apoptosis.Mol. Cell. Biochem.20113511-2415810.1007/s11010‑010‑0709‑x 21210296
     [Google Scholar]
  18. OltvalZ.N. MillimanC.L. KorsmeyerS.J. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death.Cell199374460961910.1016/0092‑8674(93)90509‑O 8358790
     [Google Scholar]
  19. KellyP.N. StrasserA. The role of Bcl-2 and its pro-survival relatives in tumourigenesis and cancer therapy.Cell Death Differ.20111891414142410.1038/cdd.2011.17 21415859
     [Google Scholar]
  20. ReedJ.C. Bcl-2 family proteins.Oncogene199817253225323610.1038/sj.onc.1202591 9916985
     [Google Scholar]
  21. BroughtonB.R.S. ReutensD.C. SobeyC.G. Apoptotic mechanisms after cerebral ischemia.Stroke2009405e331e33910.1161/STROKEAHA.108.531632 19182083
     [Google Scholar]
  22. NakkaV.P. GusainA. MehtaS.L. RaghubirR. Molecular mechanisms of apoptosis in cerebral ischemia: Multiple neuroprotective opportunities.Mol. Neurobiol.200837173810.1007/s12035‑007‑8013‑9 18066503
     [Google Scholar]
  23. RadakD. KatsikiN. ResanovicI. JovanovicA. Sudar-MilovanovicE. ZafirovicS. MousadS.A. IsenovicE.R. Apoptosis and acute brain ischemia in ischemic stroke.Curr. Vasc. Pharmacol.201715211512210.2174/1570161115666161104095522 27823556
     [Google Scholar]
  24. MikhailovV. MikhailovaM. DegenhardtK. VenkatachalamM.A. WhiteE. SaikumarP. Association of bax and bak homo-oligomers in mitochondria. Bax requirement for Bak reorganization and cytochrome c release.J. Biol. Chem.200327875367537610.1074/jbc.M203392200 12454021
     [Google Scholar]
  25. KadiogluO. NassJ. SaeedM.E. SchulerB. EfferthT. Kaempferol is an anti-inflammatory compound with activity towards NF-κB pathway proteins.Anticancer Res.201535526452650 25964540
     [Google Scholar]
  26. ChenX. YangX. LiuT. GuanM. FengX. DongW. ChuX. LiuJ. TianX. CiX. LiH. WeiJ. DengY. DengX. ChiG. SunZ. Kaempferol regulates MAPKs and NF-κB signaling pathways to attenuate LPS-induced acute lung injury in mice.Int. Immunopharmacol.201214220921610.1016/j.intimp.2012.07.007 22835426
     [Google Scholar]
  27. TanakaY. FurutaA. AsanoK. KobayashiH. Modulation of Th1/Th2 cytokine balance by quercetin in vitro.Medicines 2020784610.3390/medicines7080046 32751563
     [Google Scholar]
  28. MichalskiJ. DeinzerA. StichL. ZinserE. SteinkassererA. KnippertzI. Quercetin induces an immunoregulatory phenotype in maturing human dendritic cells.Immunobiology2020225415192910.1016/j.imbio.2020.151929 32115260
     [Google Scholar]
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Jeduxiaoliu Formula can Induce Apoptosis of Lymphoma Cells In Vitro and In Vivo
Comb Chem High Throughput Screen 28, 319 (2025); https://doi.org/10.2174/0113862073271687231122064637
/content/journals/cchts/10.2174/0113862073271687231122064637
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  • Article Type:     Research Article
Keyword(s): apoptosis; Jeduxiaoliu formula; Keywords; lymphoma; NF-κB signalling pathway; RNA-Seq; TNF signalling pathway

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