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2000
Volume 14, Issue 1
  • ISSN: 2211-5501
  • E-ISSN: 2211-551X

Abstract

Introduction

Biosurfactants are the surface active reagents produced by microorganisms, which exhibit the non-toxic and augmented bioavailability potential for the decomposition of petro-based pollutants and performing microbial enhanced oil recovery (MEOR).

Methods

Thus, the present study entails the isolation of a novel biosurfactant producing bacteria in MSM medium containing crude oil as a sole carbon source. Biosurfactant screening, cultural, and statistical optimization; Plackett - Burman Design, and response surface methodology (RSM) in conjunction with the Box-Behnken experimental design was employed for examining the biosurfactant producing potential, and emulsification activity of the strain, which rendered a maximum E index as 70%, with reduced surface tension of 29 mN/m.

Results

The novel isolate was identified and named strain ANSKSLAB02 and the sequence was deposited in the NCBI-GenBank database with accession number “KU518891”.

Conclusion

The results signify that the bacterium holds the potential to burgeon in harsh conditions which is an appropriate habitat for application in MEOR, which has great potential to aid in environmental cleanup.

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/content/journals/cbiot/10.2174/0122115501370139250207053902
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Identification and Characterization of Biosurfactant-producing Novel Paenibacillus dendritiformis Strain ANSKLAB02 from Brackish River Water
Curr. Biotechnol. 14, 52 (2025); https://doi.org/10.2174/0122115501370139250207053902
/content/journals/cbiot/10.2174/0122115501370139250207053902
/content/journals/cbiot/10.2174/0122115501370139250207053902
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  • Article Type:     Research Article
Keyword(s): 16S rRNA gene sequencing; biosurfactant production; Box-Behnken experimental design; Paenibacillus dendritiformis; Petroleum degradation; Placket-Burman

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