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2000
Volume 21, Issue 10
  • ISSN: 1573-4072
  • E-ISSN: 1875-6646

Abstract

Introduction

This study explores the therapeutic potential of (), focusing on its bioactive compounds and antioxidant effects. Phytochemical analysis combined with network pharmacology was employed to investigate its multi-targeted therapeutic potential against oxidative stress.

Methods

The antioxidant activity of flower extract was determined using DPPH assays. High-performance thin-layer chromatography (HPTLC) and liquid chromatography-mass spectrometry (LC-MS) were performed to profile key bioactive constituents. Network pharmacology analysis linked to these phytochemicals was performed to determine its role in oxidative stress and associated complications.

Results

The results showed a significant DPPH activity of the flower extract of . HPTLC profiling showed several major and minor metabolites at different Rf values, while LC-MS profiling revealed quercetin, kaempferol, genestin, . as key components of the flower extract. In network pharmacology analysis, quercetin showed a significant interaction with genes like TP53, NOS2, and CAT, while kaempferol targeted SOD2 and NFE2L2, both crucial in oxidative stress regulation. The results highlight the multi-targeted approach of flower extract compounds in modulating oxidative stress pathways.

Conclusion

This comprehensive approach revealed multiple molecular pathways influenced by compounds, providing a strong foundation for their therapeutic potential in oxidative stress-related diseases.

© 2025 Bentham Science Publishers
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2025-02-06
2025-12-14

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Exploring the Therapeutic Potential of Bixa orellana L. Phytochemicals in Oxidative Stress and Complications using Network Pharmacology
Curr. Bioact. Compd. 21, E15734072350859 (2025); https://doi.org/10.2174/0115734072350859241119103503
/content/journals/cbc/10.2174/0115734072350859241119103503
/content/journals/cbc/10.2174/0115734072350859241119103503
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  • Article Type:     Research Article
Keyword(s): B. orellana; gene interaction; network pharmacology; oxidative stress; phytochemical analysis; thin layer chromatography

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