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2000
Volume 21, Issue 6
  • ISSN: 1573-4072
  • E-ISSN: 1875-6646

Abstract

Introduction

The pharmacological significance of 5-hydroxy-3,7,4'-trimethoxy flavone (5HTMF), a flavonoid isolated from L. (Capparidaceae), in mononuclear lymphocytes of patients with Rheumatoid Arthritis (RA) has been well-documented. In previous studies, the anti-inflammatory and anti-arthritis activity of CWF was reported.

Methods

However, there is a lack of information on the toxicity of 5HTMF. This study aimed to assess the embryonic toxicity of 5HTMF on zebrafish embryos, using dexamethasone as a standard for liver toxicity. At 12 hours post fertilization (hpf), no significant toxicity from 5HTMF was observed on allantoic membrane development.

Results

By 24 hpf, 20% of embryos showed minor changes in tail development at a 100 μM concentration of 5HTMF, while dexamethasone (10 μM) disrupted the allantoic membrane in 61.11% of embryos. In the liver toxicity assay, 5HTMF did not show any significant toxicity.

Conclusion

At 48 hpf, dexamethasone exhibited substantial toxicity at a concentration of 10 μM. In conclusion, the findings suggest that 5HTMF may be a safe and non-toxic dietary supplement for treating chronic inflammatory conditions such as RA.

© 2025 Bentham Science Publishers
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2025-10-29

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Safety Assessment of Cat’s Whiskers Flavonoid in Development Toxicity of Zebrafish Embryos
Curr. Bioact. Compd. 21, e15734072344456 (2025); https://doi.org/10.2174/0115734072344456241209101357
/content/journals/cbc/10.2174/0115734072344456241209101357
/content/journals/cbc/10.2174/0115734072344456241209101357
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  • Article Type:     Research Article
Keyword(s): 5-hydroxy-3,7,4̍̍-tri methoxy flavone; Cleome gynandra L; embryonic toxicity; liver toxicity; streptavidin-peroxidase assay; zebra fish

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