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2000
Volume 21, Issue 5
  • ISSN: 1573-4072
  • E-ISSN: 1875-6646

Abstract

Introduction

Non-optimized extraction conditions can lead to loss, degradation, and modification of biomolecules. In this study, we investigated the influence of various factors on the extraction yield and antioxidant activity of total carotenoids from orange coral .

Materials and Methods

Total carotenoid yields were compared using eleven different solvent systems, with acetone demonstrating the highest extraction efficiency. Subsequently, we explored the impact of extraction time, temperature, acetone concentration, liquid-matter ratio, the addition of a synthetic antioxidant (butylated hydroxytoluene), and ultrasonic power on total carotenoids' yield and antioxidant capacities, employing experimental designs based on Plackett-Burman and Box-Behnken methodologies.

Results and Discussion

Under the different extraction conditions, total carotenoid yields exhibited a remarkable range from 2.43 mg/g to 28.12 mg/g. Additionally, the antioxidant activity saw significant enhancements from 30.68% to 57.76% for the ABTS assay and from 14.34% to 44.34% for the DPPH assay, respectively. Ultrasound extraction yielded higher carotenoid levels (25.90 mg/g . 10.65 mg/g in microwaves) and better antioxidant activity (IC values: 0.19 ± 0.002 mg/mL for ABTS, 0.35 ± 0.009 mg/mL for DPPH) compared to microwave extraction. The interplay between extraction time and temperature emerged as pivotal, with increased time initially boosting yields and optimal temperatures expediting extractions within shorter durations. Higher solvent concentrations and appropriate solvent-to-sample ratios also played a significant role in enhancing carotenoid migration.

Conclusion

By optimizing extraction conditions, researchers can enhance the efficiency and effectiveness of natural product isolation processes, thus facilitating further exploration and utilization of these valuable bioactive compounds.

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Optimization of Ultrasound-assisted Extraction of Total Carotenoids from Orange Coral “Astroides calycularis” by Response Surface Methodology
Curr. Bioact. Compd. 21, E110624230927 (2025); https://doi.org/10.2174/0115734072308822240529101221
/content/journals/cbc/10.2174/0115734072308822240529101221
/content/journals/cbc/10.2174/0115734072308822240529101221
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  • Article Type:     Research Article
Keyword(s): astroides calycularis; box-behnken design; carotenoids; Orange coral; placket-burman design; ultrasound-assisted extraction

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