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2000
Volume 21, Issue 7
  • ISSN: 1573-4110
  • E-ISSN: 1875-6727

Abstract

Pharmaceutical analysis is critical in ensuring the quality and safety of drug substances and formulations. High-performance thin-layer Chromatography (HPTLC) has emerged as a powerful analytical technique in the pharmaceutical industry due to its numerous advantages, including high separation efficiency, cost-effectiveness, and ease of sample preparation. One of its variants, Reversed-Phase High-Performance Thin-Layer Chromatography (RP-HPTLC), has gained immense popularity for analyzing nonpolar and slightly polar compounds, including drugs and their metabolites. This review paper draws attention to history and the recent developments in RP-HPTLC for pharmaceutical analysis. It highlights the advantages and limitations of RP-HPTLC, discussing its applications in drug analysis, impurity determination, stability-indicating assays, and more. In this study, recent advances in RP-HPTLC instrumentation and techniques were reviewed, including hyphenated methods, such as Reversed-Phase High-Performance Thin-Layer Chromatography coupled with Mass Spectrometry and Reversed-Phase High-Performance Thin-Layer Chromatography coupled with Nuclear Magnetic Resonance. Through this comprehensive analysis, the authors aim to underscore the potential of RP-HPTLC as a reliable and efficient analytical technique in the pharmaceutical industry and shed light on future trends in this field.

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2025-10-12

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/content/journals/cac/10.2174/0115734110320008240628090739
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Chromatography Chronicles: Unveiling the Power of Reversed-phase High-performance Thin Layer Chromatography in Pharmaceutical Analysis
Current Analytical Chemistry 21, 777 (2025); https://doi.org/10.2174/0115734110320008240628090739
/content/journals/cac/10.2174/0115734110320008240628090739
/content/journals/cac/10.2174/0115734110320008240628090739
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  • Article Type:     Review Article
Keyword(s): analytical advancements; chromatographic separation; Drug analysis; eco-friendly techniques; green solvents; hyphenated methods; pharmaceutical quality assurance; polarity; reversed-phase; sustainable chromatography

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