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2000
Volume 21, Issue 8
  • ISSN: 1573-4110
  • E-ISSN: 1875-6727

Abstract

The rapid detection of food-borne pathogens is increasing to ensure the safety of consumers, as the major food-borne illnesses are caused by pathogenic bacteria. Salmonellosis caused by is one of the primary concerns in many countries. is capable of generating toxins that can produce food poisoning in the human body.

Aims

The present study aimed to investigate the prevalence of and in seafood sample using multiplex polymerase chain reaction (mPCR). The thermostable nuclease (nuc) gene of and enterotoxin (stn) gene of were used as target genes for mPCR detection.

Methods and Material

In total, 10 seafood items, including fish, crabs, and prawns, which are generally available in Indian fish markets, were selected for the present study. Samples that carried both the strains and were selected for mPCR by targeting the stn and nuc genes.

Results

Among 10 seafood samples collected, 7 of them carried strain and 5 of them carried strains. The results showed that 75% of the salmonella strains carried stn gene, and 75% of the strains carried the nuc gene.

Conclusion

This study suggests that mPCR can be used for simultaneous detection by targeting the stn gene and nuc gene of salmonella and food-borne pathogens in seafood.

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2024-07-29
2025-12-24

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Rapid and Simultaneous Detection of Salmonella Sp. and Staphylococcus aureus in Seafood by Multiplex PCR (mPCR)
Current Analytical Chemistry 21, 960 (2025); https://doi.org/10.2174/0115734110306807240724064141
/content/journals/cac/10.2174/0115734110306807240724064141
/content/journals/cac/10.2174/0115734110306807240724064141
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  • Article Type:     Research Article
Keyword(s): foodborne pathogen; multiplex PCR; PCR; rapid detection; Salmonella sp.; seafood pathogens; Staphylococcus aureus

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