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2000
Volume 21, Issue 6
  • ISSN: 1573-4110
  • E-ISSN: 1875-6727

Abstract

Background

Rodent pests cause significant damage to the national economy and human health in China. Rodent infertility control technology offers a new and less toxic approach to managing rodent pests. The use of a plant complex sterility agent has shown the potential to induce male infertility, but the molecular mechanism behind this effect remains unclear and requires further investigation.

Methods

Sixty Sprague Dawley (SD) rats were randomly assigned to control, low-dose (10 mg/kg of plant complex sterility agent containing shikonin and acetylstilbestrol), and high-dose (20 mg/kg of plant complex sterility agent) groups. The rats received intragastric administration of the agent for 30 days. Sperm count and testosterone levels were assessed to evaluate fertility. The protein interacting with C kinase 1 (PICK1) and Toll-like receptor 4 (TLR4) levels in the control group, low-dose group, and high-dose group were measured by immunohistochemistry (IHC) and Western Blotting (WB).

Results

In comparison to the control group, both the sperm concentration ( < 0.05) and the levels of testosterone ( < 0.05), PICK1 ( < 0.05), and TLR4 ( < 0.05) were significantly decreased in a dose-dependent manner in the low dose and high dose groups. These changes were confirmed through microscopy, ELISA, IHC, and WB.

Conclusion

The plant complex sterility agent demonstrates potential in reducing sperm concentration and down-regulating testosterone levels. It likely exerts its effects by impairing male fertility in rats through the inhibition of PICK1 and TLR4 protein levels.

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Molecular Analysis of PICK1 Mediating TLR4 in Testicular Cells
Current Analytical Chemistry 21, 652 (2025); https://doi.org/10.2174/0115734110303040240912062951
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  • Article Type:     Research Article
Keyword(s): infertility; male infertility; protein interacting with C kinase 1 (PICK1); Rodent pest; sperm count; testosterone levels; toll-like receptor 4 (TLR4)

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