Skip to content
2000
image of The Effect of Biosurfactant Isolated from Actinobacteria on the bfp Gene Expression of Aeromonas hydrophila, Isolated from Children's Stool Samples

Abstract

Introduction

, a rod-shaped, gram-negative bacterium, is frequently found in aquatic surroundings and additionally present in drinking water, sewage, and food sources. This microbe is gaining recognition as a potential threat to health, classifying it as an emerging pathogen. Biosurfactants are microbial-derived compounds that share hydrophilic and hydrophobic moieties that are surface active. This study aimed to investigate the effect of biosurfactant isolated from Actinobacteria on the expression of the gene of isolated from children's stool samples to 
patent the ideal method in Qom, Iran, from May 2022 to March 2023.

Materials and Methods

Actinobacteria were isolated from soil samples of the desert areas of Qom province, Iran. Biochemical and molecular tests of 16S rRNA were used to identify Actinobacteria isolates. The produced biosurfactant was investigated by methods of hemolysis, oil droplet destruction, lipase production, oil expansion, emulsifying activity, and surface tension reduction measurement. The structure of biosurfactant was investigated by Fourier transform infrared spectroscopy (FTIR) analysis, and its effect on gene expression was measured. Also, isolates of were obtained from stool samples of children referred to Hazrat Masoumeh Hospital in Qom from May 2022 to March 2023. Then, the effect of a biosurfactant isolated from Actinobacteria on the gene expression of isolates was measured by RT-PCR.

Results

Based on sequencing data, the genus with the ability to produce biosurfactant was isolated from the soil of the studied area, which could reduce the expression of the gene after treatment with biosurfactant in clinical isolates of .

Discussion

The biosurfactant-producing isolates were identified as . The results indicated that the biosurfactant significantly decreased gene expression in . This emphasizes the potential of biosurfactants to eliminate microorganisms by reducing virulence gene expression, inhibiting biofilm formation, demonstrating antimicrobial activity, and improving emulsification. The study supports the idea that biosurfactants can interfere with bacterial mechanisms that cause disease, such as biofilm formation, which is critical for pathogen persistence and resistance. Previous research also confirms the antipathogenic activity of natural isolates against .

Conclusion

The findings of the present study show that the desert soils of Qom province are a potential area for finding actinobacterial isolates with the ability to produce biosurfactants and influence the expression of pathogenic genes of clinical isolates of .

© 2025 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/biot/10.2174/0118722083366860250623142119
2025-07-09
2025-09-02

  • From This Site

    /content/journals/biot/10.2174/0118722083366860250623142119
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. El-ghareeb H.M. Zahran E. Abd-Elghany S.M. Occurrence, molecular characterization and antimicrobial resistance of pathogenic Aeromonas hydrophila from retail fish. Alex. J. Vet. Sci. 2019 62 1
    [Google Scholar]
  2. Jabaji S. Method of using biosurfactant-producing bacteria against fungal and bacterial pathogens. Patent US2023106836A1 2023
    [Google Scholar]
  3. Pessoa R.B. Oliveira W.F. Correia M.T. Fontes A. Coelho L.C. Aeromonas and human health disorders: Clinical approaches. Front. Microbiol. 2022 13 868890 10.3389/fmicb.2022.868890 35711774
    [Google Scholar]
  4. Matys J. Turska-Szewczuk A. Sroka-Bartnicka A. Role of bacterial secretion systems and effector proteins - Insights into Aeromonas pathogenicity mechanisms. Acta Biochim. Pol. 2020 67 3 283 293 10.18388/abp.2020_5410 32865955
    [Google Scholar]
  5. Fernández-Bravo A. Figueras M.J. An update on the genus Aeromonas: Taxonomy, epidemiology, and pathogenicity. Microorganisms 2020 8 1 129 10.3390/microorganisms8010129 31963469
    [Google Scholar]
  6. Cao J. Liu C. Wang Q. Investigating of type IV pili to the pathogenicity of Aeromonas schubertii. Aquaculture 2021 530 735800 10.1016/j.aquaculture.2020.735800
    [Google Scholar]
  7. Sifri Z. Chokshi A. Cennimo D. Horng H. Global contributors to antibiotic resistance. J. Glob. Infect. Dis. 2019 11 1 36 42 10.4103/jgid.jgid_110_18 30814834
    [Google Scholar]
  8. Talebi Bezmin Abadi A. Rizvanov A.A. Haertlé T. Blatt N.L. World Health Organization report: Current crisis of antibiotic resistance. Bionanoscience 2019 9 4 778 788 10.1007/s12668‑019‑00658‑4
    [Google Scholar]
  9. Salwan R. Sharma V. Molecular and biotechnological aspects of secondary metabolites in actinobacteria. Microbiol. Res. 2020 231 126374 10.1016/j.micres.2019.126374 31756597
    [Google Scholar]
  10. Al-shaibani M.M. Radin Mohamed R.M.S. Sidik N.M. Biodiversity of secondary metabolites compounds isolated from phylum actinobacteria and its therapeutic applications. Molecules 2021 26 15 4504 10.3390/molecules26154504 34361657
    [Google Scholar]
  11. Hug J.J. Bader C.D. Remškar M. Cirnski K. Müller R. Concepts and methods to access novel antibiotics from actinomycetes. Antibiotics 2018 7 2 44 10.3390/antibiotics7020044 29789481
    [Google Scholar]
  12. Joshi S. Durden D.L. Combinatorial approach to improve cancer immunotherapy: Rational drug design strategy to simultaneously hit multiple targets to kill tumor cells and to activate the immune system. J. Oncol. 2019 2019 1 1 18 10.1155/2019/5245034 30853982
    [Google Scholar]
  13. Daccò C. Girometta C. Asemoloye M. Carpani G. Picco A. Tosi S. Key fungal degradation patterns, enzymes and their applications for the removal of aliphatic hydrocarbons in polluted soils: A review. Int. Biodeterior. Biodegradation 2020 147 104866
    [Google Scholar]
  14. Nunes C.S. Malmlöf K. Enzymatic decontamination of antimicrobials, phenols, heavy metals, pesticides, polycyclic aromatic hydrocarbons, dyes, and animal waste. Enzymes in human and animal nutrition. Elsevier 2018 331 359
    [Google Scholar]
  15. Lipczynska-Kochany E. Humic substances, their microbial interactions and effects on biological transformations of organic pollutants in water and soil: A review. Chemosphere 2018 202 420 437 29579677
    [Google Scholar]
  16. Sharma V. Salwan R. Biocontrol potential and applications of Actinobacteria in agriculture. New and future developments in microbial biotechnology and bioengineering. Elsevier 2018 93 108
    [Google Scholar]
  17. Kashif A. Rehman R. Fuwad A. Current advances in the classification, production, properties and applications of microbial biosurfactants - A critical review. Adv. Colloid Interface Sci. 2022 306 102718 35714572
    [Google Scholar]
  18. Habib S.A. Abd Burghal A. Actinobacter biosurfactants producer isolated from marsh sediments in Iraq. JSFS 2023 10 1S 5133 5142
    [Google Scholar]
  19. Pourmohsen M. Shakib P. Zolfaghari M.R. The Prevalence of bla VIM, bla KPC, bla NDM, bla IMP, bla SHV, bla TEM, bla CTX-M, and class I and II integrons Genes in Aeromonas hydrophila isolated from clinical specimens of Qom, Iran. Clin. Lab. 2023 69 1 36649515
    [Google Scholar]
  20. Wang Z.J. Jin D.N. Zhou Y. Bioactivity Ingredients of Chaenomeles speciosa against Microbes: Characterization by LC-MS and Activity Evaluation. J. Agric. Food Chem. 2021 69 16 4686 4696 33876942
    [Google Scholar]
  21. Feliatra F. Batubara U.M. Nurulita Y. Lukistyowati I. Setiaji J. The potentials of secondary metabolites from Bacillus cereus SN7 and Vagococcus fluvialis CT21 against fish pathogenic bacteria. Microb. Pathog. 2021 158 105062 10.1016/j.micpath.2021.105062 34186116
    [Google Scholar]
  22. Setiaji J. Feliatra F. Teruna H.Y. Antibacterial activity in secondary metabolite extracts of heterotrophic bacteria against Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa. F1000 Res. 2020 9 1491 10.12688/f1000research.26215.1 33537126
    [Google Scholar]
  23. Mirghani R. Saba T. Khaliq H. Biofilms: Formation, drug resistance and alternatives to conventional approaches. AIMS Microbiol. 2022 8 3 239 277 10.3934/microbiol.2022019 36317001
    [Google Scholar]
  24. Nabila M.I. Kannabiran K. Biosynthesis, characterization and antibacterial activity of copper oxide nanoparticles (CuO NPs) from actinomycetes. Biocatal. Agric. Biotechnol. 2018 15 56 62 10.1016/j.bcab.2018.05.011
    [Google Scholar]
  25. Al Laham S.A. Al Fadel F.M. Antibacterial activity of various plant extracts against antibiotic-resistant Aeromonas hydrophila. Jundishapur J. Microbiol. 2014 7 7 e11370 10.5812/jjm.11370 25368797
    [Google Scholar]
  26. Abd-Elnaby H.M. Abo-Elala G.M. Abdel-Raouf U.M. Hamed M.M. Antibacterial and anticancer activity of extracellular synthesized silver nanoparticles from marine Streptomyces rochei MHM13. Egypt. J. Aquat. Res. 2016 42 3 301 312 10.1016/j.ejar.2016.05.004
    [Google Scholar]
/content/journals/biot/10.2174/0118722083366860250623142119
Loading
The Effect of Biosurfactant Isolated from Actinobacteria on the bfp Gene Expression of Aeromonas hydrophila, Isolated from Children's Stool Samples
Bentham Science Publishers ; https://doi.org/10.2174/0118722083366860250623142119
/content/journals/biot/10.2174/0118722083366860250623142119
/content/journals/biot/10.2174/0118722083366860250623142119
Loading

Data & Media loading...


  • Article Type:     Research Article
Keywords: Actinobacteria ; Aeromonas hydrophila ; Biosurfactants ; Stool samples ; bfp gene ; Microbe

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test