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2000
Volume 23, Issue 4
  • ISSN: 2211-3525
  • E-ISSN: 2211-3533

Abstract

Background

Microbes are a rich source of antibacterial and anti-insect molecules. Due to rising antibiotic and anti-insect resistance in various sectors of the society, it is important to identify new compounds that may address these issues.

Aims and Objective

This study aimed to explore the bacteria isolated from soil to identify new molecules with antibacterial and anti-insect activity. Further, the current study is aimed at testing and characterizing antimicrobial and insecticidal properties of methanolic extracts from four different soil bacteria.

Methods

This study reports the isolation and characterization of soil bacteria by morphological, biochemical, and molecular analysis. The antibacterial potential of methanolic extracts of four bacterial strains were tested using an agar well diffusion assay, along with studying their insecticidal effects on the development and survival of . Fractionation of the methanolic extract was performed by chromatography, and the separated fractions were tested for their antibacterial activity.

Results

The bacteria belong to , and species. The extract of SKS7 exhibited maximum antibacterial activity against all tested microbes, including human pathogens. Extract from the same microbe also showed maximum anti-insect activity against by significantly increasing the pupal period by as much as 80% and hence extending the time to adult emergence. Morphological abnormalities like deformed wings, deformed pupae, and failure to emerge from pupae were also observed. Purification of the extract by HPLC and gel permeation chromatography helped us to observe a low molecular weight protein that may be responsible for its antibacterial activity.

Conclusion

Methanolic extract of SKS7 contains bioactive molecules with antibacterial and anti-insect activities. Further characterization and identification of these molecules may form the basis for the development of novel antibacterial drugs and insecticidal molecules in the future.

© 2025 Bentham Science Publishers
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Antibacterial and Insecticidal Activities of Bacillus licheniformis SKS7 Methanolic Extract
Anti Infect. Agents 23, E22113525329580 (2025); https://doi.org/10.2174/0122113525329580240820060847
/content/journals/aia/10.2174/0122113525329580240820060847
/content/journals/aia/10.2174/0122113525329580240820060847
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  • Article Type:     Research Article
Keyword(s): agar well diffusion assay; Antibacterial activity; Bacillus sp.; chromatography; insecticidal activity; Spodoptera litura

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