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2000
Volume 23, Issue 4
  • ISSN: 2211-3525
  • E-ISSN: 2211-3533

Abstract

Background

Plants contain a large number of molecules with various potentials, including antifungal impact. () is a yeast that causes Cryptococcosis infection in both immunocompromised and normal individuals worldwide.

Objective

This study aimed to investigate the inhibitory impact of plant extracts on and perform purification of the most bioactive product for further chemical identification.

Methods

The anti- potential of fourteen plants extracted by using various solvents, including water, methanol, and benzene, was tested. The most promising compound with anti- was purified. The minimal inhibitory level of the purified sub-fraction as compared to fluconazole as a standard drug was determined. The chemical identification of the purified product was carried out using FTIR, 1HNMR, elemental analysis, and Mass spectroscopy.

Results

() methanolic extract has the highest antifungal impact on and showed an inhibition zone of 3.2±0.2 cm. The methanolic extract was separated into 14 fractions, where the fifth fraction showed the highest activity. It was further separated into four sub-fractions that were tested to characterize the most promising molecule with anti- impact, which was further chemically identified as 3-Ethyl-6,7-dihydroxy-2-phenyl-chromen-4-one. The purified product led to significant alterations in the ultra-structure of relative to the standard drug.

Conclusion

contains 3-ethyl, 6,7-dihydroxy-2-chromen-4-one as a promising molecule with anti- potential.

© 2025 Bentham Science Publishers
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Chemical Identification of Purified Molecule (3-ethyl-6,7-dihydroxy-2-phenyl-chromen-4-one) Prepared from Alpinia officinarum with Anti-Cryptococcus neoformans Potential
Anti Infect. Agents 23, E22113525326883 (2025); https://doi.org/10.2174/0122113525326883240819041849
/content/journals/aia/10.2174/0122113525326883240819041849
/content/journals/aia/10.2174/0122113525326883240819041849
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  • Article Type:     Research Article
Keyword(s): Antifungal; C. neoformans potential; fluconazole; inhibition zone; transmission electron microscopy; yeast

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