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2000
Volume 17, Issue 1
  • ISSN: 2772-574X
  • E-ISSN: 2772-5758

Abstract

Background

Research on non-hormonal alternatives to traditional methods of male contraception has been prompted by the growing world population as well as the need for effective and reversible treatments. This study aimed to examine the possible antifertility potential of two medicinal herbs, ., and , that have been utilized for centuries in different cultures.

Methodology

A thorough pharmacognostical analysis was carried out to identify the ingredients of these plants. The phytoconstituents from aqueous 96% methanolic leaf extract The phytoconstituents were profiled using GC-MS analysis. To investigate how these phytoconstituents interact with important proteins involved in male fertility and identify potential candidates for the development of a plant-based oral contraceptive for men, further molecular docking (receptor protein ID of 7FIH (3.20 Å), 7BPR (1.95 Å), and 2Q7J (1.90 Å) experiments were carried out.

Results

The GC-MS analysis revealed a wide variety of bioactive chemicals. Promising interactions between the discovered phytoconstituents and specific protein targets were found by the molecular docking investigation, indicating the possibility of antifertility effects.

Conclusion

The results of this study demonstrate the medicinal use of these plants and provide the basis for the development of novel male contraceptive medicines and their further experimental validation.

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2025-02-25
2026-01-07

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References

  1. BesculidesM. LaraqueF. Unintended pregnancy among the urban poor.J. Urban Health200481334034810.1093/jurban/jth122 15273260
     [Google Scholar]
  2. World Population ReviewTotal population by country.2024Available from: https://worldpopulationreview.com/countries (Accessed July 20, 2024).
     [Google Scholar]
  3. World Health Organization (WHO)Family planning/contraception methods. Geneva: World Health Organization;2023[cited 2024 Jul 20]. Available from: https://www.who.int/news-room/fact-sheets/detail/family-planning-contraception
    [Google Scholar]
  4. LevineR. LangerA. BirdsallN. MerrickG. WrightA. BloomA.B. Contraception.Disease Control Priorities in Developing Countries2nd Ed. JamisonD.T. BremanJ.G. MeashamA.R. Oxford University Press: London200610751090
     [Google Scholar]
  5. NieschlagE. Male contribution to contraception.Andrology: Male Reproductive Health and Dysfunction.ChamSpringer International Publishing202376176710.1007/978‑3‑031‑31574‑9_45
     [Google Scholar]
  6. Paz SoldanV.A. How family planning ideas are spread within social groups in rural Malawi.Stud. Fam. Plann.200435427529010.1111/j.0039‑3665.2004.00031.x 15628785
     [Google Scholar]
  7. CookR.J. FathallaM.F. Advancing reproductive rights beyond Cairo and Beijing.Int. Fam. Plan. Perspect.199622311512110.2307/2950752
     [Google Scholar]
  8. VoukingM.Z. EvinaC.D. TadenfokC.N. Male involvement in family planning decision making in sub-Saharan Africa- what the evidence suggests.Pan Afr. Med. J.20141934910.11604/pamj.2014.19.349.5090 25922638
     [Google Scholar]
  9. NkondeH. MukangaB. DakaV. Male partner influence on Women’s choices and utilisation of family planning services in Mufulira district, Zambia.Heliyon202393e1440510.1016/j.heliyon.2023.e14405 36950585
     [Google Scholar]
  10. GodswillN.O. MonicaE.A. ErhaborS.I. Family planning decision-making among young males in Southern Africa.Afr. J. Reprod. Health20232710163510.29063/ajrh2023/v27i10.2 37915011
     [Google Scholar]
  11. JoshiS.C. SharmaA. ChaturvediM. Antifertility potential of some medicinal plants in males: An overview.Int. J. Pharm. Pharm. Sci.201135204217
     [Google Scholar]
  12. LampiaoF. Complementary and alternative medicines: The herbal male contraceptives.Afr. J. Tradit. Complement. Altern. Med.201185SSuppl.273210.4314/ajtcam.v8i5S.3 22754055
     [Google Scholar]
  13. DaniyalM. AkramM. Antifertility activity of medicinal plants.J. Chin. Med. Assoc.201578738238810.1016/j.jcma.2015.03.008 25921562
     [Google Scholar]
  14. DavidM. AinQ. AhmadM. ZamanW. JahanS. A biochemical and histological approach to study antifertility effects of methanol leaf extract of Asplenium dalhousiae Hook. in adult male rats.Andrologia2019516e1326210.1111/and.13262 30838702
     [Google Scholar]
  15. DeviP. KumarP. Nidhi; Dhamija, I. Antifertility activity of medicinal plants on male and female reproduction.Int. J. Pharm. Sci. Res.201563988100110.13040/IJPSR.0975‑8232.6(3).988‑01
     [Google Scholar]
  16. KaurR. SharmaA. KumarR. KharbR. Rising trends towards herbal contraceptives.J. Nat. Prod. Plant Resour.201114512
     [Google Scholar]
  17. D’CruzS.C. VaithinathanS. JubendradassR. MathurP.P. Effects of plants and plant products on the testis.Asian J. Androl.201012446847910.1038/aja.2010.43 20562897
     [Google Scholar]
  18. EngelhardtL. PöhnlT. NeugartS. Edible wild vegetables Urtica dioica L. and Aegopodium podagraria L.-Antioxidants affected by processing.Plants20221120271010.3390/plants11202710 36297734
     [Google Scholar]
  19. SaponaroM. GiacominiI. MorandinG. CocettaV. RagazziE. OrsoG. CarnevaliI. BerrettaM. ManciniM. PaganoF. MontopoliM. Serenoa repens and Urtica dioica fixed combination: In-vitro validation of a therapy for benign prostatic hyperplasia (BPH).Int. J. Mol. Sci.20202123917810.3390/ijms21239178 33276425
     [Google Scholar]
  20. AltamimiM.A. Abu-ReidahI.M. AltamimiA. JaradatN. Hydroethanolic extract of Urtica dioica L. (Stinging Nettle) leaves as disaccharidase inhibitor and glucose transport in Caco-2 hinderer.Molecules20222724887210.3390/molecules27248872 36558005
     [Google Scholar]
  21. Sabzian-MolaeiF. Nasiri KhaliliM.A. Sabzian-MolaeiM. ShahsavaraniH. Fattah PourA. Molaei RadA. HadiA. Urtica dioica Agglutinin: A plant protein candidate for inhibition of SARS-COV-2 receptor-binding domain for control of Covid19 Infection.PLoS One2022177e026815610.1371/journal.pone.0268156 35901082
     [Google Scholar]
  22. BhusalK.K. MagarS.K. ThapaR. LamsalA. BhandariS. MaharjanR. ShresthaS. ShresthaJ. Nutritional and pharmacological importance of stinging nettle (Urtica dioica L.): A review.Heliyon202286e0971710.1016/j.heliyon.2022.e09717 35800714
     [Google Scholar]
  23. SemwalP. RaufA. OlatundeA. SinghP. ZakyM.Y. IslamM.M. KhalilA.A. AljohaniA.S.M. Al AbdulmonemW. RibaudoG. The medicinal chemistry of Urtica dioica L.: from preliminary evidence to clinical studies supporting its neuroprotective activity.Nat. Prod. Bioprospect.20231311610.1007/s13659‑023‑00380‑5 37171512
     [Google Scholar]
  24. TaheriY. QuispeC. Herrera-BravoJ. Sharifi-RadJ. EzzatS.M. MerghanyR.M. ShaheenS. AzmiL. Prakash MishraA. SenerB. KılıçM. SenS. AcharyaK. NasiriA. Cruz-MartinsN. Tsouh FokouP.V. YdyrysA. BassygarayevZ. DaştanS.D. AlshehriM.M. CalinaD. ChoW.C. Urtica dioica-derived phytochemicals for pharmacological and therapeutic applications.Evid. Based Complement. Alternat. Med.2022202213010.1155/2022/4024331 35251206
     [Google Scholar]
  25. EiseN.T. SimpsonJ.S. ThompsonP.E. VenturaS. Aqueous extracts of Urtica dioica (stinging nettle) leaf contain a P2-purinoceptor antagonist-Implications for male fertility.PLoS One2022177e027173510.1371/journal.pone.0271735 35900970
     [Google Scholar]
  26. Erfani MajdN. MoradiH.R. MoftakharP. The effects of Urtica dioica L. root extract on rat testis.Anat. Sci. J.20171428390
     [Google Scholar]
  27. MorovvatiH. NajafzadehH. RashidiK. Effect of Urtica dioica extract on histological and histometrical changes of testis of hamster after testosterone administration.Zahedan J. Res. Med. Sci.2013151148
     [Google Scholar]
  28. GolalipourM.J. Kabiri BalajadehB. GhafariS. AzarhoshR. KhoriV. Protective effect of Urtica dioica L. (Urticaceae) on morphometric and morphologic alterations of seminiferous tubules in STZ diabetic rats.Iran. J. Basic Med. Sci.2011145472477 23493848
     [Google Scholar]
  29. GhafariS. BalajadehB.K. GolalipourM.J. Effect of Urtica dioica L. (Urticaceae) on testicular tissue in STZ-induced diabetic rats.Pak. J. Biol. Sci.2011141679880410.3923/pjbs.2011.798.804 22545354
     [Google Scholar]
  30. ZhangM. WangJ. ZhuL. LiT. JiangW. ZhouJ. PengW. WuC. Zanthoxylum bungeanum Maxim. (Rutaceae): A systematic review of its traditional uses, botany, phytochemistry, pharmacology, pharmacokinetics, and toxicology.Int. J. Mol. Sci.20171810217210.3390/ijms18102172 29057808
     [Google Scholar]
  31. SikarwarR.L.S. TiwariA.P. ShuklaA.N. SikarwarP.S. Zanthoxylum armatum DC. (Rutaceae) - An endangered tree from chhattisgarh, needs conservation.Indian For.2023149888788810.36808/if/2023/v149i8/166765
     [Google Scholar]
  32. AgnihotriS. DobhalP. AshfaqullahS. ChauhanH.K. TamtaS. Review of the botany, traditional uses, pharmacology, threats and conservation of Zanthoxylum armatum (Rutaceae).S. Afr. J. Bot.202215092092710.1016/j.sajb.2022.08.038
     [Google Scholar]
  33. AndolaH.C. GuptaS. BhaskarG. Altitudinal variation in essential oil content in leaves of Zanthoxylum alatum Roxb. A high value aromatic tree from Uttarakhand.Res. J. Med. Plant20115334835110.3923/rjmp.2011.348.351
     [Google Scholar]
  34. VermaK.K. KumarB. RajH. SharmaA. A review on chemical constituents, traditional uses, pharmacological studies of Zanthoxylum armatum (Rutaceae).J. Drug Deliv. Ther.2021112-S13614210.22270/jddt.v11i2‑S.4786
     [Google Scholar]
  35. RamchandramR. AliM. MirS.R. SultanaS. Essential oil constituents and antimicrobial activity of the seeds of Zanthoxylum armatum DC from chamoli.Uttarakhand. Eur. J. Pharm. Med. Res.2020711534541
     [Google Scholar]
  36. SultanaS. AliM. MirS.R. Phytochemical investigation of the fruits of Zanthoxylum armatum DC.Eur. J. Pharm. Med. Res.2023106393397
     [Google Scholar]
  37. EkkaG. JadhavS.K. QuraishiA. An overview of genus Zanthoxylum with special reference to its herbal significance and application.Herbs Spices20201710.5772/intechopen.92459
     [Google Scholar]
  38. FamobuwaO. AdekunbiE. AkinnifesiT. HassanG. In vitro antioxidant and anti-bacterial properties of a contraceptive herbal mixture of Zanthoxylum zanthoxyloides Lam (Rutaceae) Euphorbia hirta Linn. (Euphorbiaceae) and Abrus precatorius L. (Leguminosae).J. Adv. Med. Pharm. Sci.2016711710.9734/JAMPS/2016/24422
     [Google Scholar]
  39. ShahG.M. KhanM.A. Checklist of medicinal plants of siran valley, Mansehra, Pakistan.Ethnobot. Leafl.2006106371
     [Google Scholar]
  40. DuanJ. XuP. ChengX. MaoC. CrollT. HeX. ShiJ. LuanX. YinW. YouE. LiuQ. ZhangS. JiangH. ZhangY. JiangY. XuH.E. Structures of full-length glycoprotein hormone receptor signalling complexes.Nature2021598788268869210.1038/s41586‑021‑03924‑2 34552239
     [Google Scholar]
  41. MasutaniM. SakuraiS. ShimizuT. OhtoU. Crystal structure of TEX101, a glycoprotein essential for male fertility, reveals the presence of tandemly arranged Ly6/uPAR domains.FEBS Lett.2020594183020303110.1002/1873‑3468.13875 32608065
     [Google Scholar]
  42. AskewE.B. GampeR.T.Jr StanleyT.B. FaggartJ.L. WilsonE.M. Modulation of androgen receptor activation function 2 by testosterone and dihydrotestosterone.J. Biol. Chem.200728235258012581610.1074/jbc.M703268200 17591767
     [Google Scholar]
  43. TarasevičienėŽ. VitkauskaitėM. PaulauskienėA. ČerniauskienėJ. Wild stinging nettle (Urtica dioica L.) leaves and roots chemical composition and phenols extraction.Plants202312230910.3390/plants12020309 36679022
     [Google Scholar]
  44. HaidoM.H. MattiA.H. TaherS.M. Optimization of extraction conditions of bioactive compounds from Kurdistan species Urtica dioica.Cureus2024165e6114610.7759/cureus.61146 38933631
     [Google Scholar]
  45. PathakI. RokahaS. BajracharyaK.B. Phytoconstituents and biological activities of Zanthoxylum armatum fruit extract.J. Nep. Chem. Soci.202142112513110.3126/jncs.v42i1.35363
     [Google Scholar]
  46. KabdalT. KarakotiH. UpadhyayP. KumarR. PrakashO. DhamiA. LatwalM. PandeyG. SrivastavaR.M. KumarS. RawatD.S. Phytochemical composition, in vitro bioactivity evaluation and in silico molecular docking study of fruit essential oils of Zanthoxylum armatum DC collected from Himalayan region of Uttarakhand, India.J. Asia Pac. Entomol.202326210209010.1016/j.aspen.2023.102090
     [Google Scholar]
  47. BarkatullahI. IbrarM. MuhammadN. KhanA. KhanS.A. ZafarS. JanS. RiazN. UllahZ. FarooqU. HussainJ. Pharmacognostic and phytochemical studies of Zanthoxylum armatum DC.Pak. J. Pharm. Sci.2017302429438 28649067
     [Google Scholar]
  48. OmarG.A. MohammedL.Y. Physicochemical standardization and phytochemical screening of Urtica dioica L. leaves growing in Zakho, Kurdistan region, Iraq.Sci. J. Univer. Zakho202311330631610.25271/sjuoz.2023.11.3.1069
     [Google Scholar]
  49. CoulibalyA. HemaD.M. SawadogoI. ToeM. KiendrebeogoM. NébiéR.C.H. Physico-chemical properties of Ocimum americanum L. essential oil from Burkina Faso.Int. J. Biol. Chem. Sci.202317270170910.4314/ijbcs.v17i2.33
     [Google Scholar]
  50. DhouibiR. MoallaD. KsoudaK. Ben SalemM. HammamiS. SahnounZ. ZeghalK.M. AffesH. Screening of analgesic activity of Tunisian Urtica dioica and analysis of its major bioactive compounds by GCMS.Arch. Physiol. Biochem.2018124433534310.1080/13813455.2017.1402352 29157001
     [Google Scholar]
  51. KayatH.P. GautamS.D. JhaR.N. GC-MS analysis of hexane extract of Zanthoxylum armatum DC.Fruits. J. Pharmacogn. Phytochem.2016525862
     [Google Scholar]
  52. WaheedA. MahmudS. AkhtarM. NazirT. Studies on the components of essential oil of Zanthoxylum Armatum by GC-MS.Am. J. Anal. Chem.20112225826110.4236/ajac.2011.22031
     [Google Scholar]
  53. Muhammad IbrarB. Chemical composition and biological screening of essential oils of Zanthoxylum armatum DC leaves.J. Clin. Toxicol.201441100017210.4172/2161‑0495.1000172
     [Google Scholar]
  54. AdamsR.P. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry.5th edGruver, TX, USATexensis Publishing2017444445
     [Google Scholar]
  55. KregielD. PawlikowskaE. AntolakH. Urtica spp.: Ordinary plants with extraordinary properties.Molecules2018237166410.3390/molecules23071664 29987208
     [Google Scholar]
  56. VajićU.J. Grujić-MilanovićJ. ŽivkovićJ. ŠavikinK. GođevacD. MiloradovićZ. BugarskiB. Mihailović-StanojevićN. Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology.Ind. Crops Prod.20157491291710.1016/j.indcrop.2015.06.032
     [Google Scholar]
  57. LachguerK. ZakritiA. ChafikE.Z. Insecticidal effect of some moroccan plant extracts on Macrosiphum rosae and Gynaikothrips ficorum.J. Pharmacogn. Phytochem.2021105118124
     [Google Scholar]
  58. StanojevićL.P. StankovićM.Z. CvetkovićD.J. CakićM.D. IlićD.P. NikolićV.D. StanojevićJ.S. The effect of extraction techniques on yield, extraction kinetics, and antioxidant activity of aqueous-methanolic extracts from nettle (Urtica dioica L.) leaves.Sep. Sci. Technol.201651111817182910.1080/01496395.2016.1178774
     [Google Scholar]
  59. RawatG. BametaA. Phytochemical determination and antibacterial activity of Urtica dioica leave extracts against isolated food borne bacteria.Int. J. Curr. Microbiol. Appl. Sci.20198121704171210.20546/ijcmas.2019.812.205
     [Google Scholar]
  60. JoshiB.C. MukhijaM. KaliaA.N. Pharmacognostical review of Urtica dioica L.Int. J. Green Pharm.201484223229
     [Google Scholar]
  61. SaklaniS. ChandraS. In vitro antimicrobial activity, nutritional profile, and phytochemical screening of garhwal himalaya medicinal plant-Urtica dioica.Int. J. Pharm. Sci. Rev. Res.20121225760
     [Google Scholar]
  62. RepajićM. CeglediE. ZorićZ. PedisićS. Elez GarofulićI. RadmanS. PalčićI. Dragović-UzelacV. Bioactive compounds in wild nettle (Urtica dioica L.) leaves and stalks: Polyphenols and pigments upon seasonal and habitat variations.Foods202110119010.3390/foods10010190 33477689
     [Google Scholar]
  63. YongE.L. LoyC.J. SimK.S. Androgen receptor gene and male infertility.Hum. Reprod. Update2003911710.1093/humupd/dmg003 12638777
     [Google Scholar]
  64. GiwercmanA. KledalT. SchwartzM. GiwercmanY.L. LeffersH. ZazziH. WedellA. SkakkebækN.E. Preserved male fertility despite decreased androgen sensitivity caused by a mutation in the ligand-binding domain of the androgen receptor gene.J. Clin. Endocrinol. Metab.20008562253225910.1210/jcem.85.6.6626 10852459
     [Google Scholar]
  65. Juel MortensenL. LorenzenM. JørgensenA. AlbrethsenJ. JørgensenN. MøllerS. AnderssonA.M. JuulA. Blomberg JensenM. Possible relevance of soluble luteinizing hormone receptor during development and adulthood in boys and men.Cancers (Basel)2021136132910.3390/cancers13061329 33809538
     [Google Scholar]
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Pharmacognostic, GC-MS, and In silico Molecular Docking Studies of Phytoconstituents from Urtica dioica and Zanthoxylum armatum for Male Antifertility Potential
Recent Pat Food Nutr Agric 17, 82 (2026); https://doi.org/10.2174/012772574X356434250128112359
/content/journals/rafna/10.2174/012772574X356434250128112359
/content/journals/rafna/10.2174/012772574X356434250128112359
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  • Article Type:     Research Article
Keyword(s): contraception; family planning; GC-MS analysis; male antifertility; molecular docking; phytoconstituents; Urtica dioica; Zanthoxylum armatum

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