Seed Protein Variation Among Pepper (Capsicum Sp.) Genotypes Revealed By Maldi-Tof Analysis

Marco A. Caldas de Pinho Pessoa Filho; Carlos Bloch Junior; Danilo Fernandes da Silva Filho; Alexsandro Sobreira Galdino; Rodrigo Maranguape Silva da Cunha; Maria A. O. Alves; Thalles Barbosa Grangeiro

doi:10.2174/0929866043478482

ISSN: 0929-8665
E-ISSN: 1875-5305

Seed Protein Variation Among Pepper (Capsicum Sp.) Genotypes Revealed By Maldi-Tof Analysis
Authors: Marco A. Caldas de Pinho Pessoa Filho¹, Carlos Bloch Junior², Danilo Fernandes da Silva Filho³, Alexsandro Sobreira Galdino⁴, Rodrigo Maranguape Silva da Cunha⁵, Maria A. O. Alves⁶ and Thalles Barbosa Grangeiro⁷
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¹ Laboratorio de Citogenetica e Genetica Molecular, Depto. de Biologia, bloco 906, Campus do Pici, Universidade Federal do Ceara (UFC), Av. Humberto Monte s/n, 60.451-970; Fortaleza-CE, Brasil. ² Laboratorio de Citogenetica e Genetica Molecular, Depto. de Biologia, bloco 906, Campus do Pici, Universidade Federal do Ceara (UFC), Av. Humberto Monte s/n, 60.451-970; Fortaleza-CE, Brasil. ³ Laboratorio de Citogenetica e Genetica Molecular, Depto. de Biologia, bloco 906, Campus do Pici, Universidade Federal do Ceara (UFC), Av. Humberto Monte s/n, 60.451-970; Fortaleza-CE, Brasil. ⁴ Laboratorio de Citogenetica e Genetica Molecular, Depto. de Biologia, bloco 906, Campus do Pici, Universidade Federal do Ceara (UFC), Av. Humberto Monte s/n, 60.451-970; Fortaleza-CE, Brasil. ⁵ Laboratorio de Citogenetica e Genetica Molecular, Depto. de Biologia, bloco 906, Campus do Pici, Universidade Federal do Ceara (UFC), Av. Humberto Monte s/n, 60.451-970; Fortaleza-CE, Brasil. ⁶ Laboratorio de Citogenetica e Genetica Molecular, Depto. de Biologia, bloco 906, Campus do Pici, Universidade Federal do Ceara (UFC), Av. Humberto Monte s/n, 60.451-970; Fortaleza-CE, Brasil. ⁷ Laboratorio de Citogenetica e Genetica Molecular, Depto. de Biologia, bloco 906, Campus do Pici, Universidade Federal do Ceara (UFC), Av. Humberto Monte s/n, 60.451-970; Fortaleza-CE, Brasil.
Source: Protein and Peptide Letters, Volume 11, Issue 1, Feb 2004, p. 57 - 62
DOI: https://doi.org/10.2174/0929866043478482
- Available online: 01 Feb 2004

Abstract

A method for seed proteome analysis using MALDI-TOF mass spectrometry is described. The data were used to estimate the genetic diversity degree among twelve genotypes of pepper (Capsicum). The resulting spectra were converted into a binary matrix consisting of 23 protein data sets, and genetic similarity values were calculated with the FreeTree software and Jaccard's coefficient of similarity. We have also been able to identify the presence of certain proteins in the extracts, by checking their masses on online databases.

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2004-02-01

2025-09-18

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Article Type: Review Article

Keyword(s): capsicum; genetic variability; germplasm; mass spectrometry; time of flight

Seed Protein Variation Among Pepper (Capsicum Sp.) Genotypes Revealed By Maldi-Tof Analysis

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