Expression And Secretion Of Functional Recombinant 1 Scu-Pa:Av In Insect Cell Using Signal Peptides

Yuhui Huang; Ruiyang Tian; Wei Hu; Xiangfu Wu; Shengli Yang; Yi Gong

doi:10.2174/0929866043478455

ISSN: 0929-8665
E-ISSN: 1875-5305

Expression And Secretion Of Functional Recombinant 1 Scu-Pa:Av In Insect Cell Using Signal Peptides
Authors: Yuhui Huang¹, Ruiyang Tian², Wei Hu³, Xiangfu Wu⁴, Shengli Yang⁵ and Yi Gong⁶
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¹ Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, P.R.China , Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, P.R.China. ² Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, P.R.China , Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, P.R.China. ³ Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, P.R.China , Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, P.R.China. ⁴ Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, P.R.China , Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, P.R.China. ⁵ Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, P.R.China , Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, P.R.China. ⁶ Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, P.R.China , Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, P.R.China.
Source: Protein and Peptide Letters, Volume 11, Issue 1, Feb 2004, p. 49 - 55
DOI: https://doi.org/10.2174/0929866043478455
- Available online: 01 Feb 2004

Abstract

A fusion protein (scu-PA:AV) was expressed in the baculovirus expression system and secreted from Sf9 cells lead by signal peptides, 2mel and 3egt. The scu-PA:AV displays both the urokinase activity and membrane binding activity of its parental components. Our work indicated that it is possible to be developed as a thrombus-targeting drug.

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2004-02-01

2025-09-18

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Article Type: Review Article

Keyword(s): annexin v; baculovirus-insect cell system; low molecular weight urokinase; protein expression; signal peptide

Expression And Secretion Of Functional Recombinant 1 Scu-Pa:Av In Insect Cell Using Signal Peptides

Abstract

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