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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Purification and Characterization of a new Lectin from the Red Marine Alga Hypnea Musciformis
- Source: Protein and Peptide Letters, Volume 9, Issue 2, Apr 2002, p. 159 - 165
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- 01 Apr 2002
Abstract
A lectin from the red marine alga Hypnea musciformis (HML) was purified by extraction with 20 mM PBS, precipitation with 70% saturated ammonium sulphate, ion-exchange DEAE-Cellulose chromatography and RP-HPLC. The 9.3 kDa polypeptide agglutinates erythrocytes from various sources and shows oligomerization tendencies under certain MALDI-TOF / MS conditions. Preliminary N-terminal sequencing and biological assays strongly suggest that the HML may belong to a new class of algae lectins.
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