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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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One-Step Affinity Purification of Human Mu-Opioid Receptor Overexpressed in Baculovirus System
- Source: Protein and Peptide Letters, Volume 8, Issue 4, Aug 2001, p. 265 - 272
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- 01 Aug 2001
Abstract
Affinity chromatography on Ni-nitrilotriacetic acid agarose was used to purify human mu-opioid receptors overexpressed in Sf9 cells. We introduced a tag of 6 consecutive histidines at its carboxyl terminus for easy purification of recombinant proteins. The binding activity and identification of the purified receptor were determined by receptor binding assay, SDS-PAGE and Western blotting analysis. This procedure used in the paper offer an easy and fast route to the purification of recombinant human mu-opioid receptors.
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