Cross-Neutralizing Human Monoclonal Anti-HIV-1 Antibody 2F5: Preparation and Crystallographic Analysis of the Free and Epitope-Complexed Forms of its F ab Fragment

Steve Bryson; Annie Cunningham; Jason Ho; Rosemary C. Hynes; David E. Isenman; Brian H. Barber; Renate Kunert; Hermann Katinger; Michel Klein; Emil F. Pai

doi:10.2174/0929866013409201

ISSN: 0929-8665
E-ISSN: 1875-5305

Cross-Neutralizing Human Monoclonal Anti-HIV-1 Antibody 2F5: Preparation and Crystallographic Analysis of the Free and Epitope-Complexed Forms of its F ab Fragment
Authors: Steve Bryson¹, Annie Cunningham², Jason Ho³, Rosemary C. Hynes⁴, David E. Isenman⁵, Brian H. Barber⁶, Renate Kunert⁷, Hermann Katinger⁸, Michel Klein⁹ and Emil F. Pai¹⁰
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¹ Departments of Biochemistry, Immunology, Medical Biophysics and Molecular and Medical Genetics and Protein Engineering Network - Centres of Excellence, University of Toronto,Canada ² Departments of Biochemistry, Immunology, Medical Biophysics and Molecular and Medical Genetics and Protein Engineering Network - Centres of Excellence, University of Toronto,Canada ³ Departments of Biochemistry, Immunology, Medical Biophysics and Molecular and Medical Genetics and Protein Engineering Network - Centres of Excellence, University of Toronto,Canada ⁴ Departments of Biochemistry, Immunology, Medical Biophysics and Molecular and Medical Genetics and Protein Engineering Network - Centres of Excellence, University of Toronto,Canada ⁵ Departments of Biochemistry, Immunology, Medical Biophysics and Molecular and Medical Genetics and Protein Engineering Network - Centres of Excellence, University of Toronto,Canada ⁶ Departments of Biochemistry, Immunology, Medical Biophysics and Molecular and Medical Genetics and Protein Engineering Network - Centres of Excellence, University of Toronto,Canada ⁷ Departments of Biochemistry, Immunology, Medical Biophysics and Molecular and Medical Genetics and Protein Engineering Network - Centres of Excellence, University of Toronto,Canada ⁸ Departments of Biochemistry, Immunology, Medical Biophysics and Molecular and Medical Genetics and Protein Engineering Network - Centres of Excellence, University of Toronto,Canada ⁹ Departments of Biochemistry, Immunology, Medical Biophysics and Molecular and Medical Genetics and Protein Engineering Network - Centres of Excellence, University of Toronto,Canada ¹⁰ Departments of Biochemistry, Immunology, Medical Biophysics and Molecular and Medical Genetics and Protein Engineering Network - Centres of Excellence, University of Toronto,Canada
Source: Protein and Peptide Letters, Volume 8, Issue 5, Oct 2001, p. 413 - 418
DOI: https://doi.org/10.2174/0929866013409201
- Available online: 01 Oct 2001

Abstract

The human monoclonal antibody 2F5 is a potent neutralizer of most clades of HIV-1 and possesses protective effects against viral transmission. It recognizes the linear epitope ELDKWAS of the viral envelope protein gp41. As structural information about epitope recognition may help to develop an HIV-1 vaccine we initiated crystallographic analyses of mAb 2F5 and its epitope complex. We now report the preparation of the corresponding Fab fragments, complexation with the epitope peptide, and crystallization of free mAb 2F5 Fab as well as the peptide complex. Both crystal forms are well suited for high-resolution structural analysis.

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2001-10-01

2025-09-01

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Article Type: Review Article

Keyword(s): CROSS-NEUTRALIZING; CRYSTALLOGRAPHIC ANALYSIS; peptide complex

Cross-Neutralizing Human Monoclonal Anti-HIV-1 Antibody 2F5: Preparation and Crystallographic Analysis of the Free and Epitope-Complexed Forms of its F ab Fragment

Abstract

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