A New Validated HPLC-DAD Method for Estimation of Solasodine from Solanum nigrum Linn. Extracts and Formulations

A new, simple, accurate and precise HPLC-DAD method has been developed and validated for quantitative determination of solasodine in aerial parts of Solanum nigrum Linn. The column used was C18 (150 mm X 4.6 mm I.D., 5 μm) and mobile phase used was acetonitrile: 10 mM ammonium acetate buffer pH 7.7(65:35,v/v).The flow rate of mobile phase and detection wavelength were kept at 2.0 ml/ min and at 205 nm, respectively. The column oven temperature was set at 40 °C. The linearity of the method was found over a range of 300-1500 µg/ml with LOD and LOQ of 100 µg/ml and 300 µg/ml, respectively. The method has shown recoveries of solasodine in the range from 100.12 % -106.67 %, with low intra and inter-day % R.S.D. (0.89% and 1.25%, respectively). The method was successfully applied for quantitative evaluation of three extracts and different Ayurvedic formulations for the content of solasodine.