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2000
Volume 15, Issue 8
  • ISSN: 2210-3155
  • E-ISSN: 2210-3163

Abstract

Background

The demand for Linn-based products is increasing due to their well-known therapeutic properties for treating various diseases, such as typhus, herpes, eczema, constipation, diarrhoea, gastroenteritis, and fungal skin diseases.

Objective

This study aims to compare the total yield of the extract, bioactive compounds, and antioxidant activity of leaves using various extraction methods.

Methods

Extractions were performed using conventional maceration, Soxhlet, and non-conventional Subcritical Carbon Dioxide Soxhlet (SCDS) methods. The antioxidant properties of the leaf extracts were determined by the DPPH radical scavenging activities.

Results

Conventional methods yield higher percentages of extracts than SCDS, ranging from 1.20% to 25.14%. Soxhlet extraction with methanol showed the highest yields (25.14%) compared to hexane extraction (11.24%), indicating the solvent's influence on extraction efficiency. The effect of the sample-to-soaking solvent ratio on the extracted yield varied from 1.20 to 2.45% in the SCDS method. GCMS analysis showed the presence of 18 compounds, with phytol acetate, dihydroactinidiolide, and hexadecenoic acid methyl ester being the dominant ones. The DPPH assay shows that the extracts have antioxidant activity. The SCDS extract with methanol as a solvent had the strongest potency with lowest IC value (0.693 ± 0.1725 mg/mL), while the extract with ethanol as a soaking solvent had the weakest potency (55.56 ± 2.29 mg/mL).

Conclusion

Although traditional techniques produced greater extract percentages, the SCDS method demonstrated potential for maintaining the bioactive components of leaf extracts. Overall, this research highlights the potential of the SCDS as an alternative extraction technique to maintain the quality of bioactive compounds in plant extracts.

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2024-07-25
2025-09-06

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Extraction of Bioactive Compounds and Evaluation of Antioxidant Activity from Senna alata Linn Leaves using Subcritical Carbon Dioxide Soxhlet and Conventional Methods
Natural Products Journal, The 15, E250724232281 (2025); https://doi.org/10.2174/0122103155299703240521060228
/content/journals/npj/10.2174/0122103155299703240521060228
/content/journals/npj/10.2174/0122103155299703240521060228
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  • Article Type:     Research Article
Keyword(s): antioxidant activities; bioactive compounds; maceration; Senna alata linn; soxhlet method; subcritical carbon dioxide soxhlet (SCDS)

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