Exploring Protein-Protein Interactions with Synthetic Peptide Arrays

Development of array technologies started in the late 1980s, and today this technique has become a powerful tool for high throughput approaches in biology and chemistry. Progress was mainly driven by the human genome project and associated with the development of several new technologies, leading to the birth of additional “omic” topics such as proteomics, glycomics, or antibodyomics. In this review we focus strictly on peptide arrays applied to investigate protein-protein interactions, starting from single events up to complete interaction networks (interactomes). The specificities and interaction networks of protein interaction domains (PIDs) are our central theme. Picking practical examples from the literature, we review some general concepts about peptide arrays on planar supports for mapping protein-protein interactions.