s One New C21 Steroidal Glycosides from the Leaves of Cynanchum hancockianum

C21 steroids glycosides of various deoxysugars have been emerging from genus of Cynanchum. Besides classical C21 glycosides, many C21 glycosides with seco-ring aglycones attracted the extensive attention of many researchers. We conducted a systematic chemical investigation on Cynanchum hancockianum, which led to the isolation of one new seco-ring C21 steroidal glycosides. The new compound was identified as cynaascyriogenin 3-O-β-D-glucopyranosyl-(1-4)-α-L-cymaropyranosy-(1-4)- β-D-cymaropyranosyl-(1-4)-β-D-cymaropyranoside. The cytotoxic activities of the compound were evaluated using the A549 and MCF-7 cell lines, respectively. The IC50 values of the compound were 41.3 and 67.1μM, respectively. This is the first report on the isolation of seco-ring C21 steroidal glycosides from Cynanchum hancockianum.