Chemical Constituents of Pinus dalatensis Ferré Wood and their Effect on Proliferation of Acute Myeloid Leukemia Cells

Background: In many countries, Pinus species have an important role in economic, trade, culture and traditional medicine. Pinus plants also display diverse bioactivities. Pinus dalatensis Ferré is an endemic species in Vietnam and until now its chemical constituents have not been investigated. Therefore, this study aims to investigate the phytochemistry and antiproliferative activity of isolated compounds from Pinus dalatensis Ferré wood on acute myeloid leukemia (OCI-AML) cells. Methods: Compounds from plant material were isolated by using column chromatography. Their structures were determined by combining spectral analysis and comparison with reported data. Antiproliferative activity in OCI-AML cell line of isolated compounds was carried out by flow cytometry analysis. Results: Fifteen compounds were obtained including two diterpenoids (1, 2), five flavonoids (3, 4, 5, 6, 7), five stilbenoids (8, 9, 10, 11, 12), two lignans (13, 14) and one phenol (15). Compounds 2, 3, and 9 had inhibitory effects on the growth of OCI-AML. Conclusion: This is the first report on the chemical constituents of wood of this endemic species in Vietnam and their biological effects. Moreover, (E)-piceid and vanillic acid 4-β-D-glucopyranoside were isolated from Pinus for the first time.