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Some new substituted isatin (2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl)thiosemicarbazones were synthesized by reaction between 2,3,4,6-tetra-O-acetyl-bgr;-D-glucopyranosyl thiosemicarbazide and corresponding substituted isatins using conventional and microwave-assisted heating method. It is shown that the latter gave higher yields of products.